Peripheral Blood B-Lymphocytes Are Involved in Lymphocystis Disease Virus Infection in Flounder ( Paralichthys olivaceus ) via Cellular Receptor-Mediated Mechanism
Previous studies imply that peripheral blood leukocytes (PBLs) may play an important role in systemic lymphocystis disease virus (LCDV) dissemination, but whether the PBLs are susceptible and permissive to LCDV infection and the dissemination mechanism need to be clarified. In this study, LCDV was f...
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Published in | International journal of molecular sciences Vol. 23; no. 16; p. 9225 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
17.08.2022
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Previous studies imply that peripheral blood leukocytes (PBLs) may play an important role in systemic lymphocystis disease virus (LCDV) dissemination, but whether the PBLs are susceptible and permissive to LCDV infection and the dissemination mechanism need to be clarified. In this study, LCDV was firstly confirmed to infect the PBLs in flounder (
) in vivo, and to replicate in PBLs in vitro. Subsequently, the 27.8 kDa receptor protein (27.8R), a functional receptor mediating LCDV infection in flounder gill cells, was shown to locate on the cell membrane of PBLs and co-localize with LCDV in PBLs, while blocking of the 27.8R via pre-incubation of anti-27.8R MAb with the PBLs could obviously inhibit LCDV infection, revealing the 27.8R as a receptor for LCDV entry into PBLs. Multicolor fluorescence imaging studies verified that IgM
and IgD
B-lymphocyte were involved in LCDV infection. In the sorted IgM
B-cells, 27.8R
and LCDV
signals were simultaneously observed, and LCDV copy numbers increased with time, indicating that IgM
B-cells expressed the 27.8R and were permissive to LCDV infection. Furthermore, the dynamic changes of IgM
, 27.8R
, LCDV
and LCDV
/IgM
PBLs were monitored during the early phase of LCDV infection. It was found that the percentage of IgM
B-cells in PBLs clearly declined first and then increased, suggesting LCDV infection facilitated damage to B-cells, whereas the amounts of 27.8R
and LCDV
PBLs, as well as LCDV-infected IgM
B-cells, showed an opposite trend. These results proved that IgM
B-lymphocytes could be infected by LCDV via a receptor-mediated mechanism and support viral replication, which provided novel insights for the first time into the role of B-lymphocytes in LCDV dissemination and pathogenesis in teleost fish. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms23169225 |