The Molecular Floodgates of Stress-Induced Senescence Reveal Translation, Signalling and Protein Activity Central to the Post-Mortem Proteome

• Published in: International journal of molecular sciences Vol. 21; no. 17; p. 6422
• Main Authors: Wasinger, Valerie C, Curnoe, Darren, Boel, Ceridwen, Machin, Naomi, Goh, Hsiao Mei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.09.2020; MDPI
• Subjects: Aging; Animals; Apoptosis; Binding sites; Bioavailability; Cancer; Cardiovascular disease; Catalytic activity; Cell death; Cellular Senescence; coordinated gene-expression; Coronary artery disease; Death; Embryogenesis; Energy; Enzymatic activity; Enzyme activity; Epigenetics; Floodgates; Gene expression; Heart diseases; Inflammatory response; Kinases; Lipids; Male; Mandible; Mapping; Mass spectrometry; Mice; Mitochondria; paleoproteomics; Phenotype; Phenotypes; Phosphorylation; post-mortem; Postmortem Changes; Protein synthesis; Proteins; Proteome - analysis; Proteome - metabolism; Proteomes; Proteomics; ribosomal binding proteins; Scientific imaging; Senescence; Signal Transduction; Stress, Physiological; Translation; senescence; coordinated gene-expression; death; ribosomal binding proteins; inflammation; paleoproteomics; mitochondrial dysfunction; post-mortem
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21176422

The transitioning of cells during the systemic demise of an organism is poorly understood. Here, we present evidence that organismal death is accompanied by a common and sequential molecular flood of stress-induced events that propagate the senescence phenotype, and this phenotype is preserved in the proteome after death. We demonstrate activation of "death" pathways involvement in diseases of ageing, with biochemical mechanisms mapping onto neurological damage, embryonic development, the inflammatory response, cardiac disease and ultimately cancer with increased significance. There is sufficient bioavailability of the building blocks required to support the continued translation, energy, and functional catalytic activity of proteins. Significant abundance changes occur in 1258 proteins across 1 to 720 h post-mortem of the 12-week-old mouse mandible. Protein abundance increases concord with enzyme activity, while mitochondrial dysfunction is evident with metabolic reprogramming. This study reveals differences in protein abundances which are akin to states of stress-induced premature senescence (SIPS). The control of these pathways is significant for a large number of biological scenarios. Understanding how these pathways function during the process of cellular death holds promise in generating novel solutions capable of overcoming disease complications, maintaining organ transplant viability and could influence the findings of proteomics through "deep-time" of individuals with no historically recorded cause of death.