Biodegradable Scaffolds for Vascular Regeneration Based on Electrospun Poly(L-Lactide- co -Glycolide)/Poly(Isosorbide Sebacate) Fibers

• Published in: International journal of molecular sciences Vol. 24; no. 2; p. 1190
• Main Authors: Śmiga-Matuszowicz, Monika, Włodarczyk, Jakub, Skorupa, Małgorzata, Czerwińska-Główka, Dominika, Fołta, Kaja, Pastusiak, Małgorzata, Adamiec-Organiściok, Małgorzata, Skonieczna, Magdalena, Turczyn, Roman, Sobota, Michał, Krukiewicz, Katarzyna
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.01.2023; MDPI
• Subjects: Angiogenesis; Biocompatibility; Biodegradability; Biodegradable materials; Biodegradation; blood vessel regeneration; Blood vessels; Electrospinning; electrospun scaffolds; Endothelial Cells; Endothelium; Fibers; Genotyping; Humans; Inflammation; PLGA; poly(isosorbide sebacate); Polylactide-co-glycolide; Polymers; Scaffolds; Seaweeds; Spectrum analysis; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Umbilical vein; poly(isosorbide sebacate); PLGA; blood vessel regeneration; electrospun scaffolds
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24021190

Vascular regeneration is a complex process, additionally limited by the low regeneration potential of blood vessels. Hence, current research is focused on the design of artificial materials that combine biocompatibility with a certain rate of biodegradability and mechanical robustness. In this paper, we have introduced a scaffold material made of poly(L-lactide- -glycolide)/poly(isosorbide sebacate) (PLGA/PISEB) fibers fabricated in the course of an electrospinning process, and confirmed its biocompatibility towards human umbilical vein endothelial cells (HUVEC). The resulting material was characterized by a bimodal distribution of fiber diameters, with the median of 1.25 µm and 4.75 µm. Genotyping of HUVEC cells collected after 48 h of incubations on the surface of PLGA/PISEB scaffolds showed a potentially pro-angiogenic expression profile, as well as anti-inflammatory effects of this material. Over the course of a 12-week-long hydrolytic degradation process, PLGA/PISEB fibers were found to swell and disintegrate, resulting in the formation of highly developed structures resembling seaweeds. It is expected that the change in the scaffold structure should have a positive effect on blood vessel regeneration, by allowing cells to penetrate the scaffold and grow within a 3D structure of PLGA/PISEB, as well as stabilizing newly-formed endothelium during hydrolytic expansion.