Cannabinoid Type 1 Receptor is Undetectable in Rodent and Primate Cerebral Neural Stem Cells but Participates in Radial Neuronal Migration

• Published in: International journal of molecular sciences Vol. 21; no. 22; p. 8657
• Main Authors: Morozov, Yury M, Mackie, Ken, Rakic, Pasko
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.11.2020; MDPI
• Subjects: Abnormalities; Amino acids; Animal models; Animals; Antibodies; Cannabinoid CB1 receptors; Cannabinoids; Cannabis; CB1 receptor; Cell adhesion & migration; Cell cycle; Cell Movement; Cell Proliferation; Cerebral cortex; electron microscopic 3D reconstruction; Embryogenesis; Embryos; endocannabinoids; Gene expression; IHC data interpretation; Labeling; Macaca mulatta; Mice; Mice, Knockout; Microscopy; Molecular modelling; Monkeys; Neural stem cells; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Neurogenesis; neuron migration; Neurons; Neurons - cytology; Neurons - metabolism; Receptor, Cannabinoid, CB1 - genetics; Receptor, Cannabinoid, CB1 - metabolism; Recreational use; Stem cell transplantation; Stem cells; Substrates; Subventricular zone; neurogenesis; endocannabinoids; IHC data interpretation; CB1 receptor; neuron migration; electron microscopic 3D reconstruction
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21228657

Cannabinoid type 1 receptor (CB R) is expressed and participates in several aspects of cerebral cortex embryonic development as demonstrated with whole-transcriptome mRNA sequencing and other contemporary methods. However, the cellular location of CB R, which helps to specify molecular mechanisms, remains to be documented. Using three-dimensional (3D) electron microscopic reconstruction, we examined CB R immunolabeling in proliferating neural stem cells (NSCs) and migrating neurons in the embryonic mouse ( ) and rhesus macaque ( ) cerebral cortex. We found that the mitotic and postmitotic ventricular and subventricular zone (VZ and SVZ) cells are immunonegative in both species while radially migrating neurons in the intermediate zone (IZ) and cortical plate (CP) contain CB R-positive intracellular vesicles. CB R immunolabeling was more numerous and more extensive in monkeys compared to mice. In CB R-knock out mice, projection neurons in the IZ show migration abnormalities such as an increased number of lateral processes. Thus, in radially migrating neurons CB R provides a molecular substrate for the regulation of cell movement. Undetectable level of CB R in VZ/SVZ cells indicates that previously suggested direct CB R-transmitted regulation of cellular proliferation and fate determination demands rigorous re-examination. More abundant CB R expression in monkey compared to mouse suggests that therapeutic or recreational cannabis use may be more distressing for immature primate neurons than inferred from experiments with rodents.