Transcriptome Profiling Reveals Effects of Drought Stress on Gene Expression in Diploid Potato Genotype P3-198

• Published in: International journal of molecular sciences Vol. 20; no. 4; p. 852
• Main Authors: Yang, Xiaohui, Liu, Jie, Xu, Jianfei, Duan, Shaoguang, Wang, Qianru, Li, Guangcun, Jin, Liping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.02.2019; MDPI
• Subjects: Abscisic acid; Breeding; Cultivars; Dehydration; Deoxyribonucleic acid; diploid; Diploids; Diploidy; DNA; Drought; Drought resistance; Droughts; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Gene Ontology; Genomes; Genotype; Genotypes; Kinases; Molecular modelling; Nucleotide sequence; Osmosis; Phenotype; Phosphotransferase; Polyethylene Glycols - pharmacology; potato; Potatoes; Protein kinase; Protein Kinases - metabolism; Proteins; Reproducibility of Results; response gene; Solanum tuberosum - genetics; Solanum tuberosum - physiology; Stress, Physiological - genetics; Transcription Factors - metabolism; diploid; drought; potato; response gene
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20040852

Potato ( L.) is one of the three most important food crops worldwide; however, it is strongly affected by drought stress. The precise molecular mechanisms of drought stress response in potato are not very well understood. The diploid potato genotype P3-198 has been verified to be highly resistant to drought stress. Here, a time-course experiment was performed to identify drought resistance response genes in P3-198 under polyethylene glycol (PEG)-induced stress using RNA-sequencing. A total of 1665 differentially expressed genes (DEGs) were specifically identified, and based on gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the transcription factor activity, protein kinase activity, and the plant hormone signal transduction process were significantly enriched. Annotation revealed that these DEGs mainly encode transcription factors, protein kinases, and proteins related to redox regulation, carbohydrate metabolism, and osmotic adjustment. In particular, genes encoding abscisic acid (ABA)-dependent signaling molecules were significantly differentially expressed, which revealed the important roles of the ABA-dependent signaling pathway in the early response of P3-198 to drought stress. Quantitative real-time PCR experimental verification confirmed the differential expression of genes in the drought resistance signaling pathway. Our results provide valuable information for understanding potato drought-resistance mechanisms, and also enrich the gene resources available for drought-resistant potato breeding.