The Exoproteome of Staphylococcus pasteuri Isolated from Cervical Mucus during the Estrus Phase in Water Buffalo ( Bubalus bubalis )

• Published in: Biomolecules (Basel, Switzerland) Vol. 12; no. 3; p. 450
• Main Authors: Srinivasan, Mahalingam, Muthukumar, Subramanian, Rajesh, Durairaj, Kumar, Vinod, Rajakumar, Rajamanickam, Akbarsha, Mohammad Abdulkader, Gulyás, Balázs, Padmanabhan, Parasuraman, Archunan, Govindaraju
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.03.2022; MDPI
• Subjects: Animals; Bacteria; Bubalus bubalis; Buffaloes; Cell culture; Cell interactions; Cervix; Cervix Mucus; chemical communication; Chromatography; Chromatography, Liquid; Commensalism; Communication; Computer applications; cpn60; Elongation factor EF-Tu; enolase; Estrus; Estrus cycle; extracellular proteins; Female; Fructose-bisphosphate aldolase; Immune system; lipoprotein; Mass spectrometry; microbes; Microbiomes; Molecular weight; Mucus; Ontology; Pathogenicity; Peptides; Pheromones; Phosphopyruvate hydratase; Proteins; Scientific imaging; Signal transduction; Staphylococcus; Staphylococcus pasteuri; Tandem Mass Spectrometry; Vagina; Volatiles; United States > US; chemical communication; extracellular proteins; enolase; microbes; cpn60; lipoprotein
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom12030450

Bacterial extracellular proteins participate in the host cell communication by virtue of the modulation of pathogenicity, commensalism and mutualism. Studies on the microbiome of cervical mucus of the water buffalo ( ) have shown the occurrence of and that the presence of this bacterium is indicative of various physiological and reproductive states in the host. Recently, has been isolated from the cervical mucus of the buffalo during the different phases of estrous cycle, and has proved to be much more pronounced during the estrus phase. The basis underlying the availability of a significantly increased population, specifically during the estrus phase, is not known. Consequently, it is important to determine the significance of the specific abundance of during the estrus phase of the buffalo host, particularly from the perspective of whether this bacterial species is capable of contributing to sexual communication via its extracellular proteins and volatiles. Therefore, the relevance of exoproteome in the buffalo cervical mucus during the estrus phase was analyzed using LC-MS/MS. As many as 219 proteins were identified, among which elongation factor Tu (EF-Tu), 60-kDa chaperonin (Cpn60), enolase, fructose-bisphosphate aldolase class 1 (FBP aldolase), enoyl-[acyl-carrier-protein] reductase [NADPH] (ENR) and lipoprotein (Lpp) were the functionally important candidates. Most of the proteins present in the exoproteome of were those involved in cellular-metabolic functions, as well as catalytic- and binding activities. Moreover, computational studies of Lpp have shown enhanced interaction with volatiles such as acetic-, butanoic-, isovaleric- and valeric acids, which were identified in the cervical mucus . culture supernatant. The present findings suggest that extracellular proteins may play an important role in buffalo sexual communication during the estrus phase.