Genome-Wide Identification and Analysis of the MYB Transcription Factor Gene Family in Chili Pepper ( Capsicum spp.)

• Published in: International journal of molecular sciences Vol. 22; no. 5; p. 2229
• Main Authors: Arce-Rodríguez, Magda L, Martínez, Octavio, Ochoa-Alejo, Neftalí
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.02.2021; MDPI
• Subjects: Ascorbic acid; Biosynthesis; Capsaicin; capsaicinoids; Capsicum; Capsicum - genetics; Capsicum annuum; Cell cycle; chili pepper; Chromosomes; Circadian rhythm; Deoxyribonucleic acid; DNA; expression profiles; Flowers & plants; Gene expression; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genome-Wide Association Study; Genomes; Lignin; Localization; Metabolism; Multigene Family; MYB transcription factor; Peppers; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Protein structure; Proteins; Proto-Oncogene Proteins c-myb - genetics; Proto-Oncogene Proteins c-myb - metabolism; secondary metabolism; Sequence analysis; Transcription factors; capsaicinoids; carotenoids; vitamin C; expression profiles; lignins; secondary metabolism; Capsicum; MYB transcription factor; chili pepper; phenylpropanoids
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22052229

The MYB transcription factor family is very large and functionally diverse in plants, however, only a few members of this family have been reported and characterized in chili pepper ( spp.). In the present study, we performed genome-wide analyses of the MYB family in , including phylogenetic relationships, conserved domain, gene structure organization, motif protein arrangement, chromosome distribution, chemical properties predictions, RNA-seq expression, and RT-qPCR expression assays. A total of 235 non-redundant MYB proteins were identified from , including R2R3-MYB, 3R-MYB, atypical MYB, and MYB-related subclasses. The sequence analysis of CaMYBs compared with other plant MYB proteins revealed gene conservation, but also potential specialized genes. Tissue-specific expression profiles showed that genes were differentially expressed, suggesting that they are functionally divergent. Furthermore, the integration of our data allowed us to propose strong candidates to be regulating phenylpropanoid, lignin, capsaicinoid, carotenoid, and vitamin C biosynthesis, providing new insights into the role of MYB transcription factors in secondary metabolism. This study adds valuable knowledge about the functions of genes in various processes in the genus.