Characterization of Five Meloidogyne incognita Effectors Associated with PsoRPM3

• Published in: International journal of molecular sciences Vol. 23; no. 3; p. 1498
• Main Authors: Pu, Wenjiang, Xiao, Kun, Luo, Sifang, Zhu, Haifeng, Yuan, Zizhen, Gao, Chaoyuan, Hu, Jianfang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.01.2022; MDPI
• Subjects: Agricultural production; Amino acids; Animals; Chromatography; Cloning; Cytoplasm; Disease Resistance; effector; Effectors; Esophagus; function; Gene amplification; Helminth Proteins - genetics; Helminth Proteins - metabolism; Host plants; Host-Parasite Interactions; Hybridization; Hypersensitive response; Immune response; Infections; Life Cycle Stages; Localization; Mass spectrometry; Meloidogyne incognita; Molecular weight; Nematodes; Pathogens; Peptides; Plant cells; Plant Proteins - metabolism; Plant resistance; Proteins; Prunus - growth & development; Prunus - metabolism; Prunus - parasitology; resistance; Scientific imaging; Sequence Analysis, DNA; Tissue Distribution; Tylenchoidea - genetics; Tylenchoidea - growth & development; Tylenchoidea - metabolism; Tylenchoidea - pathogenicity; Up-Regulation; Xinjiang wild myrobalan (Prunus sogdiana); effector; Meloidogyne incognita; Xinjiang wild myrobalan (Prunus sogdiana); resistance; function
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23031498

Root-knot nematodes (RKNs) are devastating parasites that invade thousands of plants. In this study, five RKN effectors, which might interact with resistance protein PsoRPM3, were screened and identified. In situ hybridisation results showed that MiCal, MiGST_N_4, MiEFh and MiACPS are expressed in the subventral oesophageal glands (SvG), and MiTSPc hybridization signals are found in the dorsal esophageal gland (DG) of in the pre-J2. RT-qPCR data indicated that the expression of , , , and genes are highly expressed in of pra-J2 and J3/J4 stages. The expression of increased significantly in the female stage of . Moreover, all effectors found in this study localize in the cytoplasm and nucleus when transiently expressed in plant cells. In addition, MiGST_N_4, MiEFh, MiACPS and MiTSPc can elicit the ROS burst and strong hypersensitive response (HR), as well as significant ion leakage. Our data suggest that MiGST_N_4, MiEFh, MiACPS and MiTSPc effectors may be involved in triggering the immune response of the host plant.