Identification and Functional Analysis of Tomato CIPK Gene Family

• Published in: International journal of molecular sciences Vol. 21; no. 1; p. 110
• Main Authors: Zhang, Yao, Zhou, Xi'nan, Liu, Siyuan, Yu, Anzhou, Yang, Chuanming, Chen, Xiuling, Liu, Jiayin, Wang, Aoxue
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.12.2019; MDPI
• Subjects: Abiotic stress; Amino acids; Bioinformatics; Calcineurin; Calcium; Calcium ions; Cellular stress response; cipk; Cold; Cold Temperature; Dehydration - enzymology; Dehydration - genetics; Drought resistance; Functional analysis; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Gene silencing; Genes; Kinases; Low temperature; Low temperature resistance; Multigene Family; Nucleotide sequence; Nucleotides; Physiology; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant stress; Potassium; Protein kinase; Protein Serine-Threonine Kinases - biosynthesis; Protein Serine-Threonine Kinases - genetics; Proteins; Signal transduction; slcipk1 and slcipk8; Solanum lycopersicum; Solanum lycopersicum - enzymology; Solanum lycopersicum - genetics; tomato; Tomatoes; SlCIPK1 and SlCIPK8; tomato; CIPK; abiotic stress
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21010110

The calcineurin B-like interacting protein kinase (CIPK) protein family is a critical protein family in plant signaling pathways mediated by Ca , playing a pivotal role in plant stress response and growth. However, to the best of our knowledge, no study of the tomato gene family in response to abiotic stress has been reported. In this study, 22 members of the tomato gene family were successfully identified by using a combination of bioinformatics techniques and molecular analyses. The expression level of each member of tomato gene family under abiotic stress (low temperature, high salt, drought treatment) was determined by qRT-PCR. Results indicated that tomato CIPK demonstrated different degrees of responding to various abiotic stresses, and changes in and expression level were relatively apparent. The results of qRT-PCR showed that expression levels of increased significantly in early stages of cold stress, and the expression level of increased significantly during the three treatments at different time points, implicating CIPK1(SlCIPK1) and CIPK8 (SlCIPK8) involvement in abiotic stress response. and were silenced using Virus-induced gene silencing (VIGS), and physiological indexes were detected by low temperature, drought, and high salt treatment. The results showed that plants silenced by and at the later stage of cold stress were significantly less resistant to cold than wild-type plants. and silenced plants had poor drought resistance, indicating a relationship between SlCIPK1 and SlCIPK8 with response to low temperature and drought resistance. This is the first study to uncover the nucleotide sequence for tomato CIPK family members and systematically study the changes of tomato CIPK family members under abiotic stress. Here, we investigate the CIPK family's response under abiotic stress providing understanding into the signal transduction pathway. This study provides a theoretical basis for elucidating the function of tomato at low temperature and its molecular mechanism of regulating low temperatures.