Cellular Mechanism Underlying rTMS Treatment for the Neural Plasticity of Nervous System in Drosophila Brain

• Published in: International journal of molecular sciences Vol. 20; no. 18; p. 4625
• Main Authors: Luo, Ying, Yang, Junqing, Wang, Hong, Gan, Zongjie, Ran, Donzhi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.09.2019; MDPI
• Subjects: Action Potentials; Animal models; Animals; Brain; Brain - physiology; Brain slice preparation; Calcium; calcium channel; Calcium Channels - metabolism; Dendritic structure; Drosophila; Drosophila - physiology; Drosophila Proteins - metabolism; excitatory neuronal transmission; Glomerulus; Insects; Nervous system; Neural networks; Neural plasticity; Neuronal Plasticity; Neurons; Olfactory glomeruli; Plastic foam; projection neurons; repetitive transcranial magnetic stimulation; synaptic plasticity; Transcranial magnetic stimulation; Transcranial Magnetic Stimulation - methods; calcium channel; synaptic plasticity; projection neurons; excitatory neuronal transmission; repetitive transcranial magnetic stimulation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20184625

Repetitive transcranial magnetic stimulation (rTMS) is used as a research tool and clinical treatment for the non-clinical and clinical populations, to modulate brain plasticity. In the case of neurologic and psychiatric disease, there is significant evidence to suggest that rTMS plays an important role in the functional recovery after neurological dysfunction. However, the causal role for rTMS in the recovery of nervous dysfunction remains unclear. The purpose of the present study is to detect the regulation of rTMS on the excitatory neuronal transmission and specify the mode of action of rTMS on the neural plasticity using whole brain. Therefore, we identified the effects of rTMS on the neural plasticity of central neural system (CNS) by detecting the electrophysiology properties of projection neurons (PNs) from adult brain after rTMS. Using patch clamp recordings, we recorded the mini excitatory postsynaptic current (mEPSC) of PNs after rTMS at varying frequencies (1 Hz and 100 Hz) and intensities (1%, 10%, 50%, and 100%). Then, the chronic electrophysiology recordings, including mEPSC, spontaneous action potential (sAP), and calcium channel currents from PNs after rTMS at low frequency (1 Hz), with low intensity (1%) were detected and the properties of the recordings were analyzed. Finally, the frequency and decay time of mEPSC, the resting potential and frequency of sAP, and the current density and rise time of calcium channel currents were significantly changed by rTMS. Our work reveals that rTMS can be used as a tool to regulate the presynaptic function of neural circuit, by modulating the calcium channel in a frequency-, intensity- and time-dependent manner.