Development of Atropa belladonna L. Plants with High-Yield Hyoscyamine and without Its Derivatives Using the CRISPR/Cas9 System
L. is one of the most important herbal plants that produces hyoscyamine or atropine, and it also produces anisodamine and scopolamine. However, the hyoscyamine content is very low, and it is difficult and expensive to independently separate hyoscyamine from the tropane alkaloids in . Therefore, it i...
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Published in | International journal of molecular sciences Vol. 22; no. 4; p. 1731 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
09.02.2021
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | L. is one of the most important herbal plants that produces hyoscyamine or atropine, and it also produces anisodamine and scopolamine. However, the
hyoscyamine content is very low, and it is difficult and expensive to independently separate hyoscyamine from the tropane alkaloids in
. Therefore, it is vital to develop
plants with high yields of hyoscyamine, and without anisodamine and scopolamine. In this study, we generated
plants without anisodamine and scopolamine, via the CRISPR/Cas9-based disruption of hyoscyamine 6
-hydroxylase (
), for the first time. Hyoscyamine production was significantly elevated, while neither anisodamine nor scopolamine were produced, in the
.
plants with homozygous mutations in
. In summary, new varieties of
with high yields of hyoscyamine and without anisodamine and scopolamine have great potential applicability in producing hyoscyamine at a low cost. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms22041731 |