Nano size effects of TiO2 nanotube array on the glioma cells behavior

• Published in: International journal of molecular sciences Vol. 14; no. 1; pp. 244 - 254
• Main Authors: Yang, He, Qin, Xiaofei, Tian, Ang, Zhang, Dongyong, Xue, Xiangxin, Wu, Anhua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.12.2012; MDPI
• Subjects: Animals; Apoptosis; Behavior; Brain cancer; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Gene Expression Regulation, Neoplastic - drug effects; Glioma - pathology; glioma cells; inflammatory mediator’ nanotube; Nanotubes - chemistry; Nanotubes - ultrastructure; Particle Size; proliferation; Rats; RNA, Messenger - genetics; RNA, Messenger - metabolism; s gene; signal channel; Stem cells; Thermodynamics; Titanium - pharmacology; Tumors; X-Ray Diffraction; China
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms14010244

In order to investigate the interplay between the cells and TiO(2) nanotube array, and to explore the ability of cells to sense the size change in nano-environment, we reported on the behavior of glioma C6 cells on nanotube array coatings in terms of proliferation and apoptosis. The behavior of glioma C6 cells was obviously size-dependent on the coatings; the caliber with 15 nm diameter provided effective spacing to improve the cells proliferation and enhanced the cellular activities. C6 cells' biological behaviors showed many similar tendencies to many phorocytes; the matching degree of geometry between nanotube and integrin defined that a spacing of 15 nm was optimal for inducing signals to nucleus, which results in achieving maximum activity of glioma cells. In addition, the immune behavior of cells was studied, a variety of inflammatory mediator's gene expression levels were controlled by the nanoscale dimension, the expressions of IL-6 and IL-10 were higher on 30 nm than on 15 nm nanotube.