ELDA: Extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays

• Published in: Journal of immunological methods Vol. 347; no. 1; pp. 70 - 78
• Main Authors: Hu, Yifang, Smyth, Gordon K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.2009; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Count; Clopper–Pearson; Computational Biology; Computer software; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Generalized linear models; Humans; Indicator Dilution Techniques; Internet; Least-Squares Analysis; Likelihood Functions; Likelihood ratio tests; Linear Models; Models, Biological; Molecular immunology; Poisson Distribution; Single-hit hypothesis; Software; Stem cell assays; Stem Cells; Techniques; Tumor Cells, Cultured; Single-hit hypothesis; Computer software; Clopper–Pearson; Likelihood ratio tests; Generalized linear models; Stem cell assays; Cell population; Stem cell; Clopper-Pearson; Software; Models; Immunological method
• ISSN: 0022-1759; 1872-7905; 1872-7905
• DOI: 10.1016/j.jim.2009.06.008

ELDA is a software application for limiting dilution analysis (LDA), with particular attention to the needs of stem cell assays. It is the first limiting dilution analysis software to provide meaningful confidence intervals for all LDA data sets, including those with 0% or 100% responses. Other features include a test of the adequacy of the single-hit hypothesis, tests for frequency differences between multiple data sets, and the ability to take advantage of cases where the number of cells in the sample is counted exactly. A webtool at http://bioinf.wehi.edu.au/software/elda/ provides an easy user interface.