A Novel Antibacterial Titanium Modification with a Sustained Release of Pac-525

• Published in: Nanomaterials (Basel, Switzerland) Vol. 11; no. 12; p. 3306
• Main Authors: He, Yuzhu, Li, Yuanyuan, Zuo, Enjun, Chai, Songling, Ren, Xiang, Fei, Tao, Ma, Guowu, Wang, Xiumei, Liu, Huiying
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.12.2021; MDPI
• Subjects: acid–alkali heat pretreated mineralization; Antibacterial materials; Antimicrobial activity; Antimicrobial agents; Antimicrobial peptides; Bacteria; Biocompatibility; Biomedical materials; Biomimetics; Bionics; Coating; Coatings; Coliforms; Controlled release; Cytotoxicity; Dental implants; Dental prosthetics; Dental restorative materials; E coli; electrospray; Glycolic acid; Gram-negative bacteria; Gram-positive bacteria; Hydroxyapatite; Life sciences; Microspheres; Mineralization; Morphology; Orthopedics; Pac-525; Peptides; Polylactide-co-glycolide; Reagents; Surgical implants; Sustained release; Titanium; titanium modification; Toxicity; Beijing China; United States > US; China; Japan; acid–alkali heat pretreated mineralization; titanium modification; electrospray; Pac-525
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano11123306

For the benefit of antibacterial Ti on orthopedic and dental implants, a bioactive coating (Pac@PLGA MS/HA coated Ti) was deposited on the surface of pure titanium (Ti), which included two layers: an acid-alkali heat pretreated biomimetic mineralization layer and an electrosprayed Poly (D,L-lactide-co- glycolic acid) (PLGA) microsphere layer as a sustained-release system. Hydroxyapatite (HA) in mineralization layer was primarily prepared on the Ti followed by the antibacterial coating of Pac-525 loaded by PLGA microspheres. After observing the antimicrobial peptides distributed uniformly on the titanium surface, the release assay showed that the release of Pac-525 from Pac@PLGA MS/HA coated Ti provided a large initial burst followed by a slow release at a flat rate. Pac@PLGA MS/HA coated Ti exhibited a strong cytotoxicity to both Gram-negative bacteria ( ) and Gram-positive bacteria ( ). In addition, Pac@PLGA MS/HA coated Ti did not affect the growth and adhesion of the osteoblast-like cell line, MC3T3-E1. These data suggested that a bionic mineralized composite coating with long-term antimicrobial activity was successfully prepared.