Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation

• Published in: International journal of molecular sciences Vol. 19; no. 2; p. 638
• Main Authors: Huang, Fan-Chen, Fu, Bi-Ju, Liu, Yin-Tzu, Chang, Yao-Ren, Chi, Shin-Fei, Chien, Pei-Ru, Huang, Si-Chi, Hwang, Hau-Hsuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.02.2018; MDPI
• Subjects: Agrobacterium; Agrobacterium - physiology; Arabidopsis - genetics; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Crown gall; Deoxyribonucleic acid; Disease Susceptibility; DNA; DNA, Bacterial - genetics; Flowers; Gall; Gene Expression; Gene Expression Regulation, Plant; Host-Pathogen Interactions; Infections; Leaves; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mutation; Organ Specificity; Plant bacterial diseases; Plant cells; Plant Diseases - genetics; Plant Diseases - microbiology; Plants (botany); Plants, Genetically Modified; Protein Binding; Proteins; Recombinant Fusion Proteins; Rosette; RTNLB; Seedlings; T-DNA; Ti plasmid; Transcription; Transformation, Genetic; Transgenic plants; Virulence; Yeasts - genetics; Yeasts - metabolism; RTNLB; Agrobacterium
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19020638

can genetically transform various eukaryotic cells because of the presence of a resident tumor-inducing (Ti) plasmid. During infection, a defined region of the Ti plasmid, transfer DNA (T-DNA), is transferred from bacteria into plant cells and causes plant cells to abnormally synthesize auxin and cytokinin, which results in crown gall disease. T-DNA and several virulence (Vir) proteins are secreted through a type IV secretion system (T4SS) composed of T-pilus and a transmembrane protein complex. Three members of reticulon-like B (RTNLB) proteins, RTNLB1, 2, and 4, interact with VirB2, the major component of T-pilus. Here, we have identified that other RTNLB proteins, RTNLB3 and 8, interact with VirB2 in vitro. Root-based transformation assays with , or single mutants showed that the mutant was resistant to infection. In addition, and mutants showed reduced transient transformation efficiency in seedlings. or overexpression transgenic plants showed increased susceptibility to and infection. and transcript levels differed in roots, rosette leaves, cauline leaves, inflorescence, flowers, and siliques of wild-type plants. Taken together, and may participate in infection but may have different roles in plants.