A Highly Expressed Antennae Odorant-Binding Protein Involved in Recognition of Herbivore-Induced Plant Volatiles in Dastarcus helophoroides

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3464
• Main Authors: Yi, Shan-Cheng, Wu, Yu-Hang, Yang, Rui-Nan, Li, Dong-Zhen, Abdelnabby, Hazem, Wang, Man-Qun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.02.2023; MDPI
• Subjects: Adults; Animals; Antennae; Arthropod Antennae - metabolism; behaviorally attractive substances; binding affinities; Chemical ecology; Coleoptera - metabolism; Dastarcus helophoroides; Ecosystem; herbivore-induced plant volatiles; Herbivores; Herbivory; Host plants; Insect Proteins - metabolism; Ligands; Molecular docking; Molecular Docking Simulation; Natural enemies; Odorant-binding protein; odorant-binding proteins; Olfactory perception; p-Cymene; Pheromones; Physiology; Proteins; Receptors, Odorant - metabolism; Recognition; RNA-mediated interference; Terpinene; Volatiles; behaviorally attractive substances; Dastarcus helophoroides; binding affinities; herbivore-induced plant volatiles; odorant-binding proteins
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043464

Natural enemies such as parasitoids and parasites depend on sensitive olfactory to search for their specific hosts. Herbivore-induced plant volatiles (HIPVs) are vital components in providing host information for many natural enemies of herbivores. However, the olfactory-related proteins involved in the recognition of HIPVs are rarely reported. In this study, we established an exhaustive tissue and developmental expression profile of odorant-binding proteins (OBPs) from , an essential natural enemy in the forestry ecosystem. Twenty DhelOBPs displayed various expression patterns in different organs and adult physiological states, suggesting a potential involvement in olfactory perception. In silico AlphaFold2-based modeling and molecular docking showed similar binding energies between six DhelOBPs (DhelOBP4, 5, 6, 14, 18, and 20) and HIPVs from . While in vitro fluorescence competitive binding assays showed only recombinant DhelOBP4, the most highly expressed in the antennae of emerging adults could bind to HIPVs with high binding affinities. RNAi-mediated behavioral assays indicated that DhelOBP4 was an essential functional protein for adults recognizing two behaviorally attractive substances: -cymene and γ-terpinene. Further binding conformation analyses revealed that Phe 54, Val 56, and Phe 71 might be the key binding sites for DhelOBP4 interacting with HIPVs. In conclusion, our results provide an essential molecular basis for the olfactory perception of and reliable evidence for recognizing the HIPVs of natural enemies from insect OBPs' perspective.