MePHD1 as a PHD-Finger Protein Negatively Regulates ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava
ADP-glucose pyrophosphorylase (AGPase) is an important enzyme in the starch synthesis pathway. Its enzyme activity can determine the efficiency of starch biosynthesis. Cassava ( Crantz) is the main staple crop worldwide and has a high starch content in its storage root. However, the inner regulatory...
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Published in | International journal of molecular sciences Vol. 19; no. 9; p. 2831 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
19.09.2018
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | ADP-glucose pyrophosphorylase (AGPase) is an important enzyme in the starch synthesis pathway. Its enzyme activity can determine the efficiency of starch biosynthesis. Cassava (
Crantz) is the main staple crop worldwide and has a high starch content in its storage root. However, the inner regulatory mechanism of AGPase gene family is unclear.
; a plant homeodomain transcription factor; was isolated through a yeast one-hybrid screening using the promoter of
(
) as bait, and cassava storage root cDNA library as prey. This factor could bind to the
promoter in vitro and in vivo, and its predicted binding region ranged from -400 bp to -201 bp, at the translation initiation site. The transcript level of
could be induced by five plant hormones, and a temperature of 42 °C. This was down-regulated during the maturation process of the storage root. MePHD1 protein could repress the promoter activity of
gene by a dual-luciferase assay; which indicated that MePHD1 is a negative regulator for the transcript level of
gene. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms19092831 |