MePHD1 as a PHD-Finger Protein Negatively Regulates ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava

• Published in: International journal of molecular sciences Vol. 19; no. 9; p. 2831
• Main Authors: Ma, Ping'an, Chen, Xin, Liu, Chen, Xia, Zhiqiang, Song, Yu, Zeng, Changying, Li, Youzhi, Wang, Wenquan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.09.2018; MDPI
• Subjects: Abscisic acid; ADP glucose pyrophosphorylase; Amino acids; Barley; Biosynthesis; Cassava; Down-Regulation; Enzymatic activity; Enzyme activity; Enzymes; Gene expression; Gene Expression Regulation, Plant; Genomics; Glucose; Glucose-1-Phosphate Adenylyltransferase - genetics; Glucose-1-Phosphate Adenylyltransferase - metabolism; Homeobox; Homeodomain Proteins - metabolism; Hormones; Manihot - genetics; Manihot - metabolism; Manihot esculenta; plant homeodomain transcription factor; Plant Proteins - genetics; Plant Proteins - metabolism; Prey; Promoter Regions, Genetic; Protein Binding; Proteins; Salinity; Seeds; Starch; starch biosynthesis; Transcription factors; Transcription Factors - metabolism; Translation initiation; Yeast; China; starch biosynthesis; plant homeodomain transcription factor; ADP-glucose pyrophosphorylase; cassava
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19092831

ADP-glucose pyrophosphorylase (AGPase) is an important enzyme in the starch synthesis pathway. Its enzyme activity can determine the efficiency of starch biosynthesis. Cassava ( Crantz) is the main staple crop worldwide and has a high starch content in its storage root. However, the inner regulatory mechanism of AGPase gene family is unclear. ; a plant homeodomain transcription factor; was isolated through a yeast one-hybrid screening using the promoter of ( ) as bait, and cassava storage root cDNA library as prey. This factor could bind to the promoter in vitro and in vivo, and its predicted binding region ranged from -400 bp to -201 bp, at the translation initiation site. The transcript level of could be induced by five plant hormones, and a temperature of 42 °C. This was down-regulated during the maturation process of the storage root. MePHD1 protein could repress the promoter activity of gene by a dual-luciferase assay; which indicated that MePHD1 is a negative regulator for the transcript level of gene.