The Roles of Cullins E3 Ubiquitin Ligases in the Lipid Biosynthesis of the Green Microalgae Chlamydomonas reinhardtii

• Published in: International journal of molecular sciences Vol. 22; no. 9; p. 4695
• Main Authors: Luo, Qiulan, Zou, Xianghui, Wang, Chaogang, Li, Yajun, Hu, Zhangli
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.04.2021; MDPI
• Subjects: Algae; Amino acids; Aquatic microorganisms; Biodiesel fuels; Biofuels; Biosynthesis; Chlamydomonas reinhardtii; Cullin; Diesel; Enzymes; Eukaryotes; fatty acid; Fatty acids; Gene expression; Genetic engineering; Genomes; Genomics; Glycerol; Iron; Kinases; lipid metabolic; Lipid metabolism; Lipids; Localization; Metabolism; Nitrogen; Nutrient deficiency; Nutrition; Phylogenetics; Physiological responses; Proteins; RNA inference; RNA-mediated interference; Sulfur; Ubiquitin; Ubiquitination; fatty acid; lipid metabolic; RNA inference; cullin; Chlamydomonas reinhardtii
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22094695

Microalgae-based biodiesel production has many advantages over crude oil extraction and refinement, thus attracting more and more concern. Protein ubiquitination is a crucial mechanism in eukaryotes to regulate physiological responses and cell development, which is highly related to algal biodiesel production. Cullins as the molecular base of cullin-RING E3 ubiquitin ligases (CRLs), which are the largest known class of ubiquitin ligases, control the life activities of eukaryotic cells. Here, three cullins (CrCULs) in the green microalgae were identified and characterized. To investigate the roles of CrCULs in lipid metabolism, the gene expression profiles of s under nutrition starvation were examined. Except for down-regulation under nitrogen starvation, the gene was induced by sulfur and iron starvation. seemed insensitive to nitrogen and sulfur starvation because it only had changes after treatment for eight days. exhibited an expression peak after nitrogen starvation for two days but this declined with time. All s expressions significantly increased under iron deficiency at two and four days but decreased thereafter. The silencing of and expression using RNAi (RNA interference) resulted in biomass decline and lipids increase but an increase of 20% and 28% in lipid content after growth for 10 days, respectively. In and RNAi lines, the content of fatty acids, especially C16:0 and C18:0, notably increased as well. However, the lipid content and fatty acids of the RNAi strain slightly changed. Moreover, the subcellular localization of CrCUL4 showed a nuclear distribution pattern. These results suggest CrCUL2 and CrCUL4 are regulators for lipid accumulation in . This study may offer an important complement of lipid biosynthesis in microalgae.