Fine Physical Bin Mapping of the Powdery Mildew Resistance Gene Pm21 Based on Chromosomal Structural Variations in Wheat

• Published in: International journal of molecular sciences Vol. 19; no. 2; p. 643
• Main Authors: Zhu, Shanying, Ji, Yaoyong, Ji, Jian, Bie, Tongde, Gao, Anli, He, Huagang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.02.2018; MDPI
• Subjects: Chromosomes; Cloning; comparative mapping; Gene mapping; Genes; Kinases; Mapping; Physical mapping; Plant breeding; Pm21; Powdery mildew; powdery mildew resistance; Wheat; Pm21; physical mapping; powdery mildew resistance; comparative mapping
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19020643

, derived from wheat wild relative , is one of the most effective powdery mildew resistance genes and has been widely applied in wheat breeding in China. Mapping and cloning are of importance for understanding its resistance mechanism. In the present study, physical mapping was performed using different genetic stocks involving in structural variations of chromosome 6VS carrying . The data showed that 6VS could be divided into eight distinguishable chromosomal bins, and was mapped to the bin FLb4-b5/b6 closely flanked by the markers 6VS-08.6 and 6VS-10.2. Comparative genomic mapping indicated that the orthologous regions of FLb4-b5/b6 carrying were narrowed to a 117.7 kb genomic region harboring 19 genes in and a 37.7 kb region harboring 5 genes in rice, respectively. The result was consistent with that given by recent genetic mapping in diploid . In conclusion, this study demonstrated that physical mapping based on chromosomal structural variations is an efficient method for locating alien genes in wheat background.