Phage S144, A New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

• Published in: International journal of molecular sciences Vol. 21; no. 15; p. 5196
• Main Authors: Gambino, Michela, Nørgaard Sørensen, Anders, Ahern, Stephen, Smyrlis, Georgios, Gencay, Yilmaz Emre, Hendrix, Hanne, Neve, Horst, Noben, Jean-Paul, Lavigne, Rob, Brøndsted, Lone
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.07.2020; MDPI
• Subjects: Antigens; Bacteria; Bacteriophages - chemistry; Bacteriophages - genetics; Bacteriophages - metabolism; Bacteriophages - ultrastructure; Biosynthesis; C-Terminus; Classification; Corticoviridae - genetics; Cronobacter sakazakii; Cronobacter sakazakii - genetics; Cronobacter sakazakii - virology; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA, Viral - genetics; E coli; Enterobacteriaceae; Fibers; Genes; Genome, Viral; Genomes; Genomics; Host Specificity; Infections; Lipopolysaccharides; Microscopy, Electron, Transmission; Morphogenesis; Nucleotides; O Antigens - genetics; O Antigens - metabolism; Open Reading Frames; phage; Phages; Plaques; polyvalent; Proteins; Proteomics; Resistant mutant; RNA polymerase; Salmonella; Salmonella - genetics; Salmonella - virology; Salmonella Phages - genetics; Sequence Analysis, DNA; Structural proteins; Tail fiber protein; Tandem Mass Spectrometry; Transmission electron microscopy; tRNA; phage; polyvalent; Enterobacteriaceae
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21155196

Phages are generally considered species- or even strain-specific, yet polyvalent phages are able to infect bacteria from different genera. Here, we characterize the novel polyvalent phage S144, a member of the genus. By screening 211 strains, we found that phage S144 forms plaques on specific serovars of subsp. and on . Analysis of phage resistant mutants suggests that the O-antigen of lipopolysaccharide is the phage receptor in both bacterial genera. The S144 genome consists of 53,628 bp and encodes 80 open reading frames (ORFs), but no tRNA genes. In total, 32 ORFs coding for structural proteins were confirmed by ESI-MS/MS analysis, whereas 45 gene products were functionally annotated within DNA metabolism, packaging, nucleotide biosynthesis and phage morphogenesis. Transmission electron microscopy showed that phage S144 is a myovirus, with a prolate head and short tail fibers. The putative S144 tail fiber structure is, overall, similar to the tail fiber of phage Mu and the C-terminus shows amino acid similarity to tail fibers of otherwise unrelated phages infecting . Since all phages in the genus encode tail fibers similar to S144, we suggest that phages in this genus infect and are polyvalent.