APE1/Ref-1 Inhibits Adipogenic Transcription Factors during Adipocyte Differentiation in 3T3-L1 Cells

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3251
• Main Authors: Lee, Eun-Ok, Joo, Hee-Kyoung, Lee, Yu-Ran, Kim, Sungmin, Lee, Kwon-Ho, Lee, Sang-Do, Jeon, Byeong-Hwa
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.02.2023; MDPI
• Subjects: 3T3-L1 Cells; Adenoviruses; adipocyte differentiation; Adipocytes; Adipocytes - metabolism; adipogenic transcription factor; Animals; APE1/Ref-1; Body fat; C/EBP-α; Cardiovascular disease; CCAAT-Enhancer-Binding Protein-alpha - metabolism; CCAAT/enhancer-binding protein; Cell Differentiation; Diabetes; Differentiation; DNA repair; Endonuclease; Gene expression; Gene regulation; Inflammation; Insulin; Metabolic syndrome; Mice; Obesity; Peroxisome proliferator-activated receptors; Peroxisome Proliferator-Activated Receptors - metabolism; PPAR gamma - metabolism; PPAR-γ; Protein expression; Proteins; Redox factor-1; Stem cells; Therapeutic targets; Transcription factors; Transcription Factors - metabolism; 3T3-L1 cells; adipogenic transcription factor; APE1/Ref-1; C/EBP-α; adipocyte differentiation; PPAR-γ
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24043251

Apurinic/apyrimidinic endonuclease 1/redox factor-1 ( ) is a multifunctional protein involved in DNA repair and redox regulation. The redox activity of is involved in inflammatory responses and regulation of DNA binding of transcription factors related to cell survival pathways. However, the effect of on adipogenic transcription factor regulation remains unknown. In this study, we investigated the effect of on the regulation of adipocyte differentiation in 3T3-L1 cells. During adipocyte differentiation, expression significantly decreased with the increased expression of adipogenic transcription factors such as CCAAT/enhancer binding protein ( )- and peroxisome proliferator-activated receptor ( )- , and the adipocyte differentiation marker adipocyte protein 2 ( ) in a time-dependent manner. However, overexpression inhibited , and expression, which was upregulated during adipocyte differentiation. In contrast, silencing or redox inhibition of using E3330 increased the mRNA and protein levels of , , and during adipocyte differentiation. These results suggest that inhibits adipocyte differentiation by regulating adipogenic transcription factors, suggesting that is a potential therapeutic target for regulating adipocyte differentiation.