Thyroid disrupting effects of halogenated and next generation chemicals on the swim bladder development of zebrafish

• Published in: Aquatic toxicology Vol. 193; pp. 228 - 235
• Main Authors: Godfrey, Amy, Hooser, Blair, Abdelmoneim, Ahmed, Horzmann, Katharine A., Freemanc, Jennifer L., Sepúlveda, Maria S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: Air Sacs - drug effects; Air Sacs - embryology; Animals; Caprylates - toxicity; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - physiology; Endocrine Disruptors - toxicity; Fish; Flame retardants; Fluorocarbons - toxicity; Halogenation; Organophosphorus Compounds - toxicity; Perfluorinated; Phenanthrenes - toxicity; Polybrominated Biphenyls - toxicity; Surfactants; Swim bladder; Thyroid Gland - drug effects; Thyroid Gland - embryology; Water Pollutants, Chemical - toxicity; Zebrafish - embryology; Zebrafish - metabolism; Flame retardants; Fish; Perfluorinated; Surfactants; Swim bladder
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2017.10.024

•Fish had deflated swim bladders after subchronic exposures to chemicals.•Chemicals increased thyroid peroxidase and surfactant protein gene expression.•Chronic exposures resulted in ∼50% of the fish with no anterior swim bladder.•Results suggest thyroid disruption by halogenated chemicals via surfactant system. Endocrine disrupting chemicals (EDCs) can alter thyroid function and adversely affect growth and development. Halogenated compounds, such as perfluorinated chemicals commonly used in food packaging, and brominated flame retardants used in a broad range of products from clothing to electronics, can act as thyroid disruptors. Due to the adverse effects of these compounds, there is a need for the development of safer next generation chemicals. The objective of this study was to test the thyroid disruption potential of old use and next generation halogenated chemicals. Zebrafish embryos were exposed to three old use compounds, perfluorooctanoic acid (PFOA), tetrabromobisphenol A (TBBPA) and tris (1,3-dichloro-2-propyl) phosphate (TDCPP) and two next generation chemicals, 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxdie (DOPO) and perfluorobutyric acid (PFBA). Sub-chronic (0–6days post fertilization (dpf)) and chronic (0–28dpf) exposures were conducted at 1% of the concentration known to kill 50% (LC50) of the population. Changes in the surface area of the swim bladder as well as in expression levels of genes involved in the thyroid control of swim bladder inflation were measured. At 6dpf, zebrafish exposed to all halogenated chemicals, both old use and next generation, had smaller posterior swim bladder and increased expression in the gene encoding thyroid peroxidase, tpo and the genes encoding two swim bladder surfactant proteins, sp-a and sp-c. These results mirrored the effects of thyroid hormone-exposed positive controls. Fish exposed to a TPO inhibitor (methimazole, MMI) had a decrease in tpo expression levels at 28dpf. Effects on the anterior swim bladder at 28dpf, after exposure to MMI as well as both old and new halogenated chemicals, were the same, i.e., absence of SB in ∼50% of fish, which were also of smaller body size. Overall, our results suggest thyroid disruption by the halogenated compounds tested via the swim bladder surfactant system. However, with the exception of TBBPA and TDCPP, the concentrations tested (∼5–137ppm) are not likely to be found in the environment.