Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator

• Published in: Journal of bioscience and bioengineering Vol. 113; no. 6; pp. 694 - 696
• Main Authors: Kato, Tatsuya, Manohar, Suganthi Lavender, Kanamasa, Shin, Ogata, Makoto, Park, Enoch Y.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2012; Elsevier
• Subjects: Animals; Baculoviridae - genetics; Baculovirus; Bioengineering; Biological and medical sciences; Biotechnology; Bombyx - genetics; Bombyx - metabolism; Bombyx - virology; Burst sequence; DNA-Binding Proteins - genetics; Fundamental and applied biological sciences. Psychology; Polyhedrin promoter; Promoter Regions, Genetic; Rats; Regulatory Sequences, Nucleic Acid; Sialyltransferases - genetics; Silkworm; silkworms; Trans-Activators - genetics; transactivators; transcription (genetics); Very late transcriptional factor-1; Viral Proteins - genetics; Very late transcriptional factor-1; Burst sequence; Polyhedrin promoter; Baculovirus; Silkworm; Leader sequence; Transcription; Insecta; Coexpression; Optimization; Virus; Baculoviridae; Arthropoda; Lepidoptera; Invertebrata; Transcription factor
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2012.01.010

Modified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat α2,6-sialyltransferase (ST6Gal I) in silkworm. Modified Ppolh of five BSs with VLF-1 coexpression yielded 2.9U/ml ST6Gal I activity and 32.5mU/mg specific activity, which was 1.7- and 2.3-fold higher, respectively compared to Ppolh.