Measuring Thiols in Single Cultivar South African Red Wines Using 4,4-Dithiodipyridine (DTDP) Derivatization and Ultraperformance Convergence Chromatography-Tandem Mass Spectrometry

Wine varietal thiols are important contributors to wine aroma. The chemical nature of thiols makes them difficult to measure due to low concentrations, high sensitivity to oxidation, and low ionization. Methods for the measurement of thiols usually consist of multiple steps of sample preparation fol...

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Bibliographic Details
Published inFoods Vol. 7; no. 9; p. 138
Main Authors Mafata, Mpho, Stander, Maria A, Thomachot, Baptiste, Buica, Astrid
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 30.08.2018
MDPI
Subjects
Aroma
Aroma compounds
Chromatography
Coffee
Conflicts of interest
Convergence
convergence chromatography
Cultivars
derivatization
DTDP
Food
Food science
Ionization
Ions
Linearity
Low concentrations
Mass spectrometry
Mass spectroscopy
Measurement methods
Organic chemistry
Oxidation
Parameter sensitivity
Sample preparation
Scientific imaging
Sensitivity
SPE
supercritical fluid chromatography (SFC)
Thiols
UPC2-MS/MS
Wine
Wines
United States > US
convergence chromatography
UPC2-MS/MS
SPE
supercritical fluid chromatography (SFC)
derivatization
DTDP
thiols
wine
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Summary:Wine varietal thiols are important contributors to wine aroma. The chemical nature of thiols makes them difficult to measure due to low concentrations, high sensitivity to oxidation, and low ionization. Methods for the measurement of thiols usually consist of multiple steps of sample preparation followed by instrumental measurement. Studies have collected large datasets of thiols in white wine but not in red wine, due to the lack of availability of suitable methods. In this study, for the first time, convergence chromatography was used to measure thiols in red wine at ultratrace levels with improved sensitivity compared to previous methods. Performance parameters (selectivity, linearity, limits of detection, precision, accuracy) were tested to demonstrate the suitability of the method for the proposed application. Red wine thiols were measured in South African Pinotage, Shiraz, and Cabernet Sauvignon wines ( = 16 each). Cultivar differentiation using the thiol profile was demonstrated.
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Current affiliation: Engineering Faculty of Life Sciences, National Polytechnic Institute of Toulouse (INP-ENSAT), 31500 Toulouse, France.
ISSN:2304-8158
2304-8158
DOI:10.3390/foods7090138