CircRNA Expression Pattern and ceRNA and miRNA-mRNA Networks Involved in Anther Development in the CMS Line of Brassica campestris

• Published in: International journal of molecular sciences Vol. 20; no. 19; p. 4808
• Main Authors: Liang, Yuwei, Zhang, Yuzhi, Xu, Liai, Zhou, Dong, Jin, Zongmin, Zhou, Huiyan, Lin, Sue, Cao, Jiashu, Huang, Li
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.09.2019; MDPI
• Subjects: Abortion; anther; Apoptosis; Brassica - genetics; Brassica campestris; Cell death; Cellulose; cerna; circrna; cms; Cytoplasm; Cytoplasmic male sterility; Fertility; Flowering; Flowers & plants; Flowers - genetics; Gene expression; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes; Genomes; Heterosis; Lipids; Male sterility; Males; MicroRNAs; MicroRNAs - genetics; miRNA; Morphology; Non-coding RNA; Pectin; Phenotype; Plant Development - genetics; Plants (botany); polima; Post-transcription; RNA, Circular - genetics; RNA, Messenger - genetics; Signal transduction; Transcriptome; Transcriptomes; whole-transcriptome sequencing; ceRNA; whole-transcriptome sequencing; Polima; anther; circRNA; Brassica campestris; cytoplasmic male sterility; CMS; miRNA
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20194808

Male-sterile plants provide an important breeding tool for the heterosis of hybrid crops, such as Brassicaceae. In the last decade, circular RNAs (circRNAs), as a novel class of covalently closed and single-stranded endogenous non-coding RNAs (ncRNAs), have received much attention because of their functions as "microRNA (miRNA) sponges" and "competing endogenous RNAs" (ceRNAs). However, the information about circRNAs in the regulation of male-sterility and anther development is limited. In this study, we established the Polima cytoplasm male sterility (CMS) line "Bcpol97-05A", and the fertile line, "Bcajh97-01B", in L. ssp. Makino, syn. ssp. , and performed RNA expression profiling comparisons between the flower buds of the sterile line and fertile line by whole-transcriptome sequencing. A total of 31 differentially expressed (DE) circRNAs, 47 DE miRNAs, and 4779 DE mRNAs were identified. By using Cytoscape, the miRNA-mediated regulatory network and ceRNA network were constructed, and the circRNA A02:23507399|23531438 was hypothesized to be an important circRNA regulating anther development at the post-transcriptional level. The gene ontology (GO) analysis demonstrated that miRNAs and circRNAs could regulate the orderly secretion and deposition of cellulose, sporopollenin, pectin, and tryphine; the timely degradation of lipids; and the programmed cell death (PCD) of tapetum cells, which play key roles in anther development. Our study revealed a new circRNA-miRNA-mRNA network, which is involved in the anther development of which enriched the understanding of CMS in flowering plants, and laid a foundation for further study on the functions of circRNAs and miRNAs during anther development.