Insulin Signaling in Intestinal Stem and Progenitor Cells as an Important Determinant of Physiological and Metabolic Traits in Drosophila

• Published in: Cells (Basel, Switzerland) Vol. 9; no. 4; p. 803
• Main Authors: Strilbytska, Olha M, Semaniuk, Uliana V, Storey, Kenneth B, Yurkevych, Ihor S, Lushchak, Oleh
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.03.2020; MDPI
• Subjects: Aging; Animals; Cell division; Digestive system; Digestive tract; Drosophila; Drosophila melanogaster - cytology; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila melanogaster - physiology; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Experiments; Feeding Behavior; Females; Food; fruit fly; Gene expression; Gene Expression Regulation; Genotype & phenotype; Glucose; Glucose - metabolism; Glycogen; Insects; Insulin; Insulin - metabolism; insulin signaling pathway; Insulin-like growth factor I; Insulin-Like Growth Factor I - metabolism; Intestine; Intestines - cytology; isc; Kinases; Life span; lifespan; Longevity - genetics; Malnutrition; Metabolism; Metabolites; midgut; Organ Specificity - genetics; Peptides - metabolism; Physiology; Progenitor cells; PTEN protein; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA-mediated interference; Signal Transduction; Starvation; Stem cells; Stem Cells - metabolism; Stress, Physiological - genetics; Sucrose; Survival Analysis; Syrups & sweeteners; Poland; fruit fly; insulin signaling pathway; lifespan; ISC; midgut; progenitor cells; metabolism
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells9040803

The insulin-IGF-1 signaling (IIS) pathway is conserved throughout multicellular organisms and regulates many traits, including aging, reproduction, feeding, metabolism, stress resistance, and growth. Here, we present evidence of a survival-sustaining role for IIS in a subset of gut cells in , namely the intestinal stem cells (ISCs) and progenitor cells. Using RNAi to knockdown the insulin receptor, we found that inhibition of IIS in ISCs statistically shortened the lifespan of experimental flies compared with non-knockdown controls, and also shortened their survival under starvation or malnutrition conditions. These flies also showed decreased reproduction and feeding, and had lower amounts of glycogen and glucose in the body. In addition, increased expression was observed for the transcripts for the insulin-like peptides , , and This may reflect increased insulin signaling in peripheral tissues supported by up-regulation of the target of the brain insulin gene ( ). In contrast, activation of IIS (via knockdown of the insulin pathway inhibitor PTEN) in intestinal stem and progenitor cells decreased fly resistance to malnutrition, potentially by affecting adipokinetic hormone signaling. Finally, knockdown to enhance IIS also activated JAK-STAT signaling in gut tissue by up-regulation of , , and genes, as well as genes encoding the EGF receptor ligands and . These results clearly demonstrate that manipulating insulin levels may be used to modulate various fly traits, which are important determinants of organismal survival.