Novel Seed Size : A Novel Seed-Developing Gene in Glycine max

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 4189
• Main Authors: Zhang, Mingxia, Dong, Rui, Huang, Penghui, Lu, Mingyang, Feng, Xianzhong, Fu, Yongfu, Zhang, Xiaomei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.02.2023; MDPI
• Subjects: Annotations; Biosynthesis; Cell division; Cell growth; Chalcone synthase; CRISPR; Deoxyribonucleic acid; DNA; DNA - metabolism; DNA helicase; Flavonoids; Gene expression; Genes; Genes, Plant; Genomes; Glycine max - genetics; Integument; Metabolism; Metabolites; Metabolomics; Mutants; Novel Seed Size (NSS); Phenotypes; Plant growth; seed coat; seed size; Seeds; Seeds - metabolism; Soybeans; T-DNA; T-DNA mutant; Transcription factors; Transcriptome; Transcriptomes; Websites; T-DNA mutant; Novel Seed Size (NSS); flavonoids; seed coat; seed size
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24044189

Soybean-seed development is controlled in multiple ways, as in many known regulating genes. Here, we identify a novel gene, ( ), involved in seed development, by analyzing a T-DNA mutant ( ). The mutant is a random mutant of the transgenic line, with phenotypes with small and brown seed coats. An analysis of the metabolomics and transcriptome combined with RT-qPCR in the seeds revealed that the brown coat may result from the increased expression of genes, while the down-regulated expression of leads to small seed size. The seed phenotypes and a microscopic observation of the seed-coat integument cells in a CRISPR/Cas9-edited mutant confirmed that the gene conferred small phenotypes of the seeds. As mentioned in an annotation on the Phytozome website, encodes a potential DNA helicase RuvA subunit, and no such genes were previously reported to be involved in seed development. Therefore, we identify a novel gene in a new pathway controlling seed development in soybeans.