CaMF2, an anther-specific lipid transfer protein (LTP) gene, affects pollen development in Capsicum annuum L

• Published in: Plant science (Limerick) Vol. 181; no. 4; pp. 439 - 448
• Main Authors: Chen, Changming, Chen, Guoju, Hao, Xuefeng, Cao, Bihao, Chen, Qinghua, Liu, Shaoqun, Lei, Jianjun
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.10.2011; Elsevier
• Subjects: Amino Acid Sequence; Amino acids; Amplified Fragment Length Polymorphism Analysis; Anther-specific; Anthers; Antigens, Plant - chemistry; Antigens, Plant - genetics; Antigens, Plant - metabolism; Base Sequence; Biological and medical sciences; Buds; calyx; CaMF2; Capsicum - anatomy & histology; Capsicum - genetics; Capsicum - growth & development; Capsicum - ultrastructure; Capsicum annuum; Capsicum annuum L; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; complementary DNA; corolla; Development; DNA; DNA, Complementary - genetics; Expressed Sequence Tags; Flowers; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene polymorphism; Gene Silencing; genes; Genes, Plant - genetics; Germination; Grain; Leaves; Lipid transfer protein; Lipid transfer proteins; LTP gene; Molecular Sequence Data; Organ Specificity - genetics; petals; pistils; Plant Infertility - genetics; Plant physiology and development; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Pollen; Pollen - genetics; Pollen - growth & development; Pollen - ultrastructure; Pollen development; pollen germination; RNA, Messenger - genetics; RNA, Messenger - metabolism; Roots; sepals; Sequence Alignment; Sequence Analysis, DNA; Sexual reproduction; Stems; Vegetative and sexual reproduction, floral biology, fructification; VIGS; Pollen development; Capsicum annuum L; Anther-specific; CaMF2; Lipid transfer protein; VIGS; Anther; Plant biology; Lipids; Protein; Vegetable crop; Gene; Dicotyledones; Angiospermae; Flavoring crop; Capsicum annuum; Spermatophyta; Pollen; Solanaceae; Microsporogenesis
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2011.07.003

Based on the gene differential expression analysis performed by cDNA-amplified fragment length polymorphism (cDNA-AFLP) in the genic male sterile–fertile line 114AB of Capsicum annuum L., a variety of differentially expressed cDNA fragments were detected in fertile or sterile lines. A transcript-derived fragment (TDF) specifically accumulated in the flower buds of fertile line was isolated, and the corresponding full-length cDNA and DNA were subsequently amplified. Bioinformatical analyses of this gene named CaMF2 showed that it encodes a lipid transfer protein with 94 amino acids. Spatial and temporal expression patterns analysis indicated that CaMF2 was an anther-specific gene and the expression of CaMF2 was detected only in flower buds at stage 3–7 of male fertile line with a peak expression at stage 4, but not detected in the roots, tender stems, fresh leaves, flower buds, open flowers, sepals, petals, anthers or pistils of male sterile line. Further, inhibition of the CaMF2 by virus-induced gene silencing (VIGS) method resulted in the low pollen germination ability and shriveled pollen grains. All these evidence showed that CaMF2 had a vital role in pollen development of C. annuum.