Overexpression of PtDXS Enhances Stress Resistance in Poplars

• Published in: International journal of molecular sciences Vol. 20; no. 7; p. 1669
• Main Authors: Wei, Hui, Movahedi, Ali, Xu, Chen, Sun, Weibo, Almasi Zadeh Yaghuti, Amir, Wang, Pu, Li, Dawei, Zhuge, Qiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.04.2019; MDPI
• Subjects: ABA; Abiotic stress; Abscisic acid; Amino acids; Biosynthesis; C-Terminus; Carotene; Carotenoids; Chlorophyll; Cloning; Domains; DXS; E coli; elicitor treatments; Genes; Glutamic acid; Lycopene; Metabolism; Peptides; Phylogenetics; Phylogeny; Populus trichocarpa; Proteins; Pyridines; Signal transduction; Transcription; Transketolase; ABA; elicitor treatments; GA; Populus trichocarpa; DXS
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20071669

1-Deoxy-d-xylulose-5-phosphate synthase (DXS) is the rate-limiting enzyme in the plastidial methylerythritol phosphate pathway (MEP). In this study, (XM_024607716.1) was isolated from . A bioinformatics analysis revealed that had high homology with the s of other plant species. expression differed among plant tissues and was highest in young leaves and lowest in roots. The recombinant protein was produced in BL21 (DE3), purified, and its activity evaluated. The purified protein was capable of catalyzing the formation of 1-deoxy-d-xylulose-5-phosphate (DXP) from glyceraldehyde-3-phosphate and pyruvate. A functional color assay in . harboring pAC-BETA indicated that encodes a functional protein involved in the biosynthesis of isoprenoid precursors. The treatment of . seedlings with 200 μM abscisic acid (ABA), 200 mM NaCl, 10% polyethylene glycol , and 2 mM H₂O₂ resulted in increased expression of . The ABA and gibberellic acid contents of the transgenic lines (Poplar Nanlin 895) were higher than wild types, suggesting that DXS is important in terpenoid biosynthesis. Overexpression of enhanced resistance to . infection. Furthermore, the transgenic lines showed decreased feeding by , supporting the notion that PtDXS is a key enzyme in terpenoid biosynthesis.