A Self-assembly Route for Double Bilayer Lipid Membrane Formation

• Published in: Chemphyschem Vol. 11; no. 3; pp. 569 - 574
• Main Authors: Han, Xiaojun, Achalkumar, Ammathnadu S., Cheetham, Matthew R., Connell, Simon D. A., Johnson, Benjamin R. G., Bushby, Richard J., Evans, Stephen D.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 22.02.2010; WILEY‐VCH Verlag; Wiley
• Subjects: bilayers; Biological and medical sciences; biomimetics; Chemistry; Colloidal state and disperse state; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General and physical chemistry; Lipid Bilayers - chemistry; lipids; Membrane physicochemistry; Membranes; Models, Biological; Molecular biophysics; Molecular Structure; self-assembly; membranes; Self assembly; self-assembly; Molecular assembly; bilayers; Lipids; Membrane; Bilayer; biomimetics
• ISSN: 1439-4235; 1439-7641
• DOI: 10.1002/cphc.200900798

Biomembranes: A new method of forming double bilayer lipid membranes uses NHS/EDC chemistry to link adjacent bilayers, both of which remain fluid. This approach provides a novel platform for the study of biomembranes, in which the components of the upper membrane are shielded from the solid substrate by a second membrane (see figure); and for studying more complex membrane protein systems which span double lipid bilayers.