Engineering New Protein-Protein Interactions on the β-Propeller Fold by Yeast Cell Surface Display

Reinventing the wheel: The β‐propeller domain folds like a wheel to provide key protein–protein interactions in the cell. Here we used high‐throughput yeast sorting to “invent” β‐propellers of new binding specificities with cellular targets.

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Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 14; no. 4; pp. 426 - 430
Main Authors Zhang, Keya, Li, Heng, Bhuripanyo, Karan, Zhao, Bo, Chen, Tiffany F., Zheng, Ning, Yin, Jun
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 04.03.2013
WILEY‐VCH Verlag
Subjects
Adaptor Proteins, Signal Transducing - chemistry
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - metabolism
Animals
beta-propeller folds
Cell Surface Display Techniques - methods
Cytoskeletal Proteins - chemistry
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
enzymes
genetic engineering
Kelch repeats
Kelch-Like ECH-Associated Protein 1
Mice
Models, Molecular
Mutation
Protein Folding
Protein Interaction Maps
Protein Structure, Secondary
Protein Structure, Tertiary
yeast-cell-surface display
Yeasts - genetics
Yeasts - metabolism
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Summary:Reinventing the wheel: The β‐propeller domain folds like a wheel to provide key protein–protein interactions in the cell. Here we used high‐throughput yeast sorting to “invent” β‐propellers of new binding specificities with cellular targets.
Bibliography:The Chicago Community Trust
Howard Hughes Medical Institute
ArticleID:CBIC201200718
ark:/67375/WNG-Q39MGV3K-S
National Institutes of Health - No. R01-CA107134
Chicago Biomedical Consortium
istex:F543BBF584D30980A7A4C4A71FA856AF7A416250
National Science Foundation - No. 1057092
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201200718