Essential pathogenic role for endogenous interferon‐gamma (IFN‐γ) during disease onset phase of murine experimental autoimmune orchitis. I. In vivo studies

• Published in: Clinical and experimental immunology Vol. 111; no. 3; pp. 513 - 520
• Main Authors: ITOH, M, YANO, A, XIE, Q, IWAHASHI, K, TAKEUCHI, Y, MERONI, P. L, NICOLETTI, F
• Format: Journal Article
• Language: English
• Published: Oxford BSL Blackwell Science Ltd 01.03.1998; Blackwell; Blackwell Science Inc
• Subjects: Animals; Antibodies, Monoclonal - pharmacology; Autoimmune Diseases - blood; Autoimmune Diseases - immunology; Autoimmune Diseases - therapy; autoimmunity; Biological and medical sciences; Disease Models, Animal; Down-Regulation; Experimental and animal immunopathology. Animal models; Hypersensitivity, Delayed - immunology; Immunization, Passive; Immunopathology; in vivo; Interferon-gamma - blood; Interferon-gamma - immunology; Interferon-gamma - physiology; interferon‐gamma; Male; Medical sciences; Mice; Mice, Inbred C3H; Orchitis - blood; Orchitis - immunology; Orchitis - therapy; Original; Spermatozoa - immunology; testis; Immunopathology; Animal model; Orchitis; Pathogenesis; Cytokine; Rodentia; Autoimmune disease; Vertebrata; Mammalia; Mouse; Animal; Male genital diseases; Testicular diseases; Gamma interferon
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1046/j.1365-2249.1998.00527.x

We previously found that immunization of CH3/He male mice with syngeneic testicular germ cells (TGC) without the aid of any adjuvants was sufficient to induce DTH to TGC and experimental autoimmune orchitis (EAO). To evaluate the role of endogenous IFN‐γ in this model, C3H/He mice immunized subcutaneously with TGC on days 0 and 14 received a single injection of anti‐murine IFN‐γ MoAb on day 15, 20 or 25. On day 45, DTH to TGC was tested, testis specimens were collected for histological examination, and blood samples collected for IFN‐γ measurement. The results showed that whilst MoAb treatment on day 15 or 25 did not influence DTH responses, EAO development, and appearance of IFN‐γ in the circulation, treatment on day 20 significantly suppressed all of them. Thus, a single injection with anti‐IFN‐γ MoAb may successfully down‐regulate testicular autoimmunity, provided that the treatment is given at an optimal time point during disease development.