A novel 45 kDa secretory protein from rat olfactory epithelium: primary structure and localisation

• Published in: FEBS letters Vol. 450; no. 1; pp. 126 - 130
• Main Authors: Merkulova, Maria I., Andreeva, Svetlana G., Shuvaeva, Tatyana M., Novoselov, Sergey V., Peshenko, Igor V., Bystrova, Marina F., Novoselov, Vladimir I., Fesenko, Evgeny E., Lipkin, Valery M.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 30.04.1999
• Subjects: 45 kDa secretory protein; Amino Acid Sequence; Animals; Base Sequence; cDNA cloning; Cloning, Molecular; DNA, Complementary - chemistry; In Situ Hybridization; Molecular Sequence Data; Olfactory epithelium; Olfactory Mucosa - chemistry; Proteins - chemistry; Proteins - genetics; Rats; Restriction Mapping; RNA, Messenger - analysis; Sequence Alignment; Transfer protein; cDNA cloning; 45 kDa secretory protein; Olfactory epithelium; Transfer protein
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(99)00470-6

cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46 026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein and the proteins involved in the transfer of hydrophobic ligands. Using in situ hybridisation, the 45 kDa protein mRNA expression was detected in the layer of supportive cells of olfactory epithelium, apical region of trachea, surface layer of the ciliated bronchial epithelium in lung and in skin epidermis.