Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D

A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mas...

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Published inFEBS letters Vol. 152; no. 1; pp. 1 - 5
Main Authors Takao, Toshifumi, Hitouji, Tetsuo, Aimoto, Saburo, Shimonishi, Yasutsugu, Hara, Saburo, Takeda, Tae, Takeda, Yoshifumi, Miwatani, Toshio
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 07.02.1983
Subjects
Amino Acid Sequence
Bacterial Toxins - isolation & purification
Chromatography
Enterotoxin
enterotoxins
Enterotoxins - isolation & purification
Escherichia coli
Escherichia coli - analysis
FAB mass spectrometry
Hot Temperature
Isolation
Mass Spectrometry
Molecular Weight
Enterotoxin
Amino acid sequence
Isolation
FAB mass spectrometry
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Summary:A heat-stable enterotoxin produced by a strain of enterotoxigenic Escherichia coli 18D was purified by ion-exchange and reversed-phase high-pressure liquid chromatography. The amino acid sequence of the purified toxin was determined by Edman-degradation and a combination of fast atom bombardment mass spectrometry and carboxypeptidase digestion to be Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80469-4