Effects of major dietary antioxidants on inflammatory markers of RAW 264.7 macrophages

• Published in: BioFactors (Oxford) Vol. 21; no. 1-4; pp. 113 - 117
• Main Authors: Jung, Won-Jin, Sung, Mi-Kyung
• Format: Journal Article
• Language: English
• Published: Amsterdam IOS Press 2004
• Subjects: Analysis of Variance; Animals; antioxidants; Antioxidants - pharmacology; beta Carotene - pharmacology; Cell Line; COX-2; Cyclooxygenase 2; Diet; Dinoprostone - metabolism; inflammation; inflammatory cytokines; iNOS; Interleukin-6 - metabolism; Lipopolysaccharides - toxicity; macrophages; Macrophages - drug effects; Macrophages - physiology; Mice; Nitric Oxide Synthase - metabolism; Nitric Oxide Synthase Type II; Prostaglandin-Endoperoxide Synthases - drug effects; Prostaglandin-Endoperoxide Synthases - metabolism; Quercetin - pharmacology; Tocopherols - pharmacology; Tumor Necrosis Factor-alpha - drug effects; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.552210122

Oxidative stresses are involoved in the process of chronic inflammatory diseases. The objective of this study was to evaluate and compare the anti‐inflammatory activity of major dietary antioxidants. Murine RAW264.7 macrophages (4 × 106 cells) were incubated in DMEM containing 10% FBS supplemented with 5 to 20 μM of α‐tocopherol, β‐carotene or quercetin for 24 hrs, and then treated with LPS for 16hrs. The medium was collected to measure TNF‐α, IL‐6, PGE2 and nitrite. Expressions of cyclooxygenase‐2(COX‐2) and inducible nitric oxide synthase(iNOS) were determined using the harvested cells. Results indicate that TNF‐α and IL‐6 accumulations were significantly reduced by 5 to 20 μM quercetin treatment, and 20 μM of α‐tocopherol treatment. Nitrite release was significantly reduced by 5 μM quercetin treatment. However, PGE2accumulation was not affected by any of the antioxidants used. Expressions of COX‐2 and iNOS were effectively reduced by 5 μM quercetin treatment. These findings indicate that dietary antioxidants possess significant anti‐inflammatory activities, and quercetin is the most potent antioxidant.