Fractionation of a silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity

• Published in: Journal of applied polymer science Vol. 102; no. 1; pp. 772 - 776
• Main Authors: Yeo, Joo-Hong, Lee, Kwang-Gill, Kweon, Hae-Yong, Woo, Soon-Ok, Han, Sang-Mi, Kim, Sung-Su, Demura, Makoto
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 05.10.2006; Wiley
• Subjects: Applied sciences; biocompatibility; Biological and medical sciences; Bombyx mori; Exact sciences and technology; fibers; high performance liquid chromatography (HPLC); Medical sciences; Natural polymers; Neuropharmacology; Neuroprotective agent; peptides; Pharmacology. Drug treatments; Physicochemistry of polymers; Proteins; Biological properties; Oxidative stress; fibers; Property composition relationship; Toxicity; Insecta; peptides; Bombyx mori; Silk; Natural fiber; proteins; Chemical composition; Fractionation; biocompatibility; Use; Neuroprotective agent; Antioxidant; Experimental study; Hydrogen Peroxides; Protein; Hydrolysis; Arthropoda; Bombycidae; Lepidoptera; Enzymatic digestion; Fibroin; Invertebrata; high performance liquid chromatography (HPLC)
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.23740

Fractionated components of Bombyx mori silk fibroin, which were hydrolyzed with protease, were prepared with a preparative‐recycling high‐performance‐liquid‐chromatography system to evaluate the protective effects of molecular‐weight‐controlled B. mori silk fibroin components on H2O2‐injured neuronal cells. Three major fractions, having molecular weights less than about 1500, were first collected with the recycling techniques. The highest protective effect of the molecular‐weight‐controlled B. mori silk fibroin components on H2O2‐injured neuronal cells was obtained when a fraction with a molecular weight of approximately 1400 was used. This protective effect of the silk fibroin hydrolysate on H2O2‐injured neuronal cells was suggested to correlate with the contents of aromatic amino acids such as tyrosine and phenylalanine. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 772–776, 2006