Bioactive hydroxyphenylpyrrole-dicarboxylic acids from a new marine Halomonas sp.: production and structure elucidation

• Published in: Applied microbiology and biotechnology Vol. 72; no. 4; pp. 816 - 822
• Main Authors: Wang, L, Groβe, T, Stevens, H, Brinkhoff, T, Simon, M, Liang, L, Bitzer, J, Bach, G, Zeeck, A, Tokuda, H, Lang, S
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.10.2006; Springer; Springer Nature B.V
• Subjects: Acids; Anticarcinogenic Agents - pharmacology; Antineoplastic Agents - pharmacology; Artificial seawater; Biochemistry; Biological and medical sciences; Biotechnology; Dicarboxylic Acids - chemistry; Dicarboxylic Acids - isolation & purification; Dicarboxylic Acids - pharmacology; Fundamental and applied biological sciences. Psychology; Halomonas; Halomonas - chemistry; Halomonas - metabolism; Marine; Marine ecology; Seawater; Studies; Yeasts; Marine environment; Bacteria; Dicarboxylic acid; Halomonas; Halomonadaceae; Production
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-006-0370-1

The new marine Halomonas sp. strain GWS-BW-H8hM (DSM 17996) was found to produce 3-(4'-hydroxyphenyl)-4-phenylpyrrole-2,5-dicarboxylic acid (HPPD-1) and 3,4-bis(4'-hydroxy- phenyl)pyrrole-2,5-dicarboxylic acid (HPPD-2). In initial cultivations using marine broth, only low contents of these compounds have been isolated. Improving the conditions and growing the strain on artificial seawater supplemented with tryptone 10 g l-¹, yeast extract 5 g l-¹, l-tyrosine 0.6 g l-¹, glycine 1 g l-¹, and glucose 6 g l⁻¹, the growth-associated HPPD-1 and HPPD-2 production of a 40-l batch cultivation reached the amounts of 47 mg l-¹ and 116 mg l-¹, respectively, after 65 h. Both compounds showed potent anti-tumor-promoting activities.