Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli

We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in v...

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Published inChemical communications (Cambridge, England) Vol. 48; no. 89; pp. 10966 - 10968
Main Authors Shi, Yanxiang, Bueno, Alejandro, van der Donk, Wilfred A
Format Journal Article
LanguageEnglish
Published England 18.11.2012
Subjects
Amino Acid Sequence
Bacteriocins - biosynthesis
Escherichia coli
Escherichia coli - metabolism
Molecular Sequence Data
Peptides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc36336d