RNA Interference (RNAi) Induced Gene Silencing: A Promising Approach of Hi-Tech Plant Breeding

RNA interference (RNAi) is a promising gene regulatory approach in functional genomics that has significant impact on crop improvement which permits down-regulation in gene expression with greater precise manner without affecting the expression of other genes. RNAi mechanism is expedited by small mo...

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Bibliographic Details
Published inInternational journal of biological sciences Vol. 10; no. 10; pp. 1150 - 1158
Main Authors Younis, Adnan, Siddique, Muhammad Irfan, Kim, Chang-Kil, Lim, Ki-Byung
Format Journal Article
LanguageEnglish
Published Australia Ivyspring International Publisher 01.01.2014
Subjects
Adaptation, Physiological - genetics
Agriculture - methods
Breeding - methods
Crops, Agricultural - genetics
Disease Resistance - genetics
Gene Expression Regulation, Plant - genetics
Models, Biological
Nematoda
Review
Ribonuclease III - genetics
RNA Interference
RNA, Small Interfering - genetics
RNA-Induced Silencing Complex - genetics
Salinity
Temperature
functional genomics
post transcriptional gene silencing
siRNA
crop improvement
biotic stress
abiotic stress
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Summary:RNA interference (RNAi) is a promising gene regulatory approach in functional genomics that has significant impact on crop improvement which permits down-regulation in gene expression with greater precise manner without affecting the expression of other genes. RNAi mechanism is expedited by small molecules of interfering RNA to suppress a gene of interest effectively. RNAi has also been exploited in plants for resistance against pathogens, insect/pest, nematodes, and virus that cause significant economic losses. Keeping beside the significance in the genome integrity maintenance as well as growth and development, RNAi induced gene syntheses are vital in plant stress management. Modifying the genes by the interference of small RNAs is one of the ways through which plants react to the environmental stresses. Hence, investigating the role of small RNAs in regulating gene expression assists the researchers to explore the potentiality of small RNAs in abiotic and biotic stress management. This novel approach opens new avenues for crop improvement by developing disease resistant, abiotic or biotic stress tolerant, and high yielding elite varieties.
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Competing Interests: The authors have declared that no competing interest exists.
ISSN:1449-2288
1449-2288
DOI:10.7150/ijbs.10452