Soft, skin-interfaced microfluidic systems with integrated immunoassays, fluorometric sensors, and impedance measurement capabilities

Soft microfluidic systems that capture, store, and perform biomarker analysis of microliter volumes of sweat, in situ, as it emerges from the surface of the skin, represent an emerging class of wearable technology with powerful capabilities that complement those of traditional biophysical sensing de...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 117; no. 45; pp. 27906 - 27915
Main Authors Kim, Sungbong, Lee, Boram, Reeder, Jonathan T., Seo, Seon Hee, Lee, Sung-Uk, Hourlier-Fargette, Aurélie, Shin, Joonchul, Sekine, Yurina, Jeong, Hyoyoung, Oh, Yong Suk, Aranyosi, Alexander J., Lee, Stephen P., Model, Jeffrey B., Lee, Geumbee, Seo, Min-Ho, Kwak, Sung Soo, Jo, Seongbin, Park, Gyungmin, Han, Sunghyun, Park, Inkyu, Jung, Hyo-Il, Ghaffari, Roozbeh, Koo, Jahyun, Braun, Paul V., Rogers, John A.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 10.11.2020
Subjects
Ascorbic acid
Bioengineering
Biomarkers
Biosensing Techniques
Biosensing Techniques - instrumentation
Cortisol
Dielectric Spectroscopy
Dielectric Spectroscopy - instrumentation
Dielectric Spectroscopy - methods
Electric Impedance
Equipment Design
Equipment Design - instrumentation
Equipment Design - methods
Fluorometry
Humans
Immunoassay
Immunoassays
Impedance measurement
Lab-On-A-Chip Devices
Life Sciences
Microfluidics
Microfluidics - methods
Monitoring
Physical Sciences
Sensors
Skin
Skin - chemistry
Sweat
Sweat - chemistry
Vitamin C
Wearable Electronic Devices
Wearable technology
sweat cortisol
galvanic skin response
epidermal devices
soft materials
healthcare
Online AccessGet full text

Cover

More Information
Summary:Soft microfluidic systems that capture, store, and perform biomarker analysis of microliter volumes of sweat, in situ, as it emerges from the surface of the skin, represent an emerging class of wearable technology with powerful capabilities that complement those of traditional biophysical sensing devices. Recent work establishes applications in the real-time characterization of sweat dynamics and sweat chemistry in the context of sports performance and healthcare diagnostics. This paper presents a collection of advances in biochemical sensors and microfluidic designs that support multimodal operation in the monitoring of physiological signatures directly correlated to physical and mental stresses. These wireless, battery-free, skin-interfaced devices combine lateral flow immunoassays for cortisol, fluorometric assays for glucose and ascorbic acid (vitamin C), and digital tracking of skin galvanic responses. Systematic benchtop evaluations and field studies on human subjects highlight the key features of this platform for the continuous, noninvasive monitoring of biochemical and biophysical correlates of the stress state.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
PMCID: PMC7668081
Author contributions: S.K., B.L., J.T.R., S.H.S., R.G., J.K., P.V.B., and J.A.R. designed research; S.K., J.T.R., S.H.S., S.-U.L., A.H.-F., J.S., H.J., Y.S.O., G.L., M.-H.S., S.S.K., S.J., G.P., S.H., I.P., H.-I.J., and J.K. performed research; S.K., B.L., J.T.R., J.S., Y.S., H.J., Y.S.O., A.J.A., S.P.L., J.B.M., G.L., M.-H.S., S.S.K., I.P., H.-I.J., R.G., J.K., P.V.B., and J.A.R. contributed new reagents/analytic tools; S.K., B.L., S.H.S., S.-U.L., A.H.-F., J.S., Y.S., Y.S.O., A.J.A., S.P.L., J.B.M., G.L., M.-H.S., S.S.K., S.J., G.P., S.H., I.P., H.-I.J., R.G., J.K., P.V.B., and J.A.R. analyzed data; and S.K., B.L., J.T.R., S.H.S., A.H.-F., Y.S., Y.S.O., A.J.A., S.P.L., J.B.M., S.J., R.G., J.K., P.V.B., and J.A.R. wrote the paper.
Edited by Klavs F. Jensen, Massachusetts Institute of Technology, Cambridge, MA, and approved September 25, 2020 (received for review June 18, 2020)
1S.K., B.L., J.T.R., and S.H.S. contributed equally to this work.
2Present address: Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, OR 97403.
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.2012700117