Mice immunized with bone marrow-derived dendritic cells stimulated with recombinant Coxiella burnetii Com1 and Mip demonstrate enhanced bacterial clearance in association with a Th1 immune response

• Published in: Vaccine Vol. 30; no. 48; pp. 6809 - 6815
• Main Authors: Xiong, Xiaolu, Meng, Yanfen, Wang, Xile, Qi, Yong, Li, Jiaming, Duan, Changsong, Wen, Bohai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 06.11.2012
• Subjects: Allergy and Immunology; Animals; antigens; Antigens, Bacterial - immunology; Bacterial Load; Bacterial Vaccines - administration & dosage; Bone marrow; CD4 antigen; CD4-positive T-lymphocytes; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; CD8-positive T-lymphocytes; CD8-Positive T-Lymphocytes - immunology; Coxiella burnetii; Coxiella burnetii - immunology; Dendritic cells; Dendritic Cells - immunology; Disease Models, Animal; Escherichia coli; Flow cytometry; Helper cells; Immune response; Infection; Interferon-gamma - secretion; Interleukin-4 - secretion; Lymphocytes T; Male; membrane proteins; Mice; Mice, Inbred BALB C; microbial load; Polymerase chain reaction; protective antigen; Protective antigens; Q fever; Q Fever - immunology; Q Fever - microbiology; Q Fever - pathology; quantitative polymerase chain reaction; Spleen; Spleen - immunology; Th1 Cells - immunology; Thioredoxin; Vaccination - methods; vaccines; marrow-derived dendritic cells; pt; Dendritic cells; post-transfer; LPS; BMDCs; WCA; Q fever; whole cell antigens; lipopolysaccharide; Coxiella burnetii; CMI; Protective antigens; cell-mediated immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2012.09.017

Highlights ► Recombinant proteins Com1, Mip and GroEL were cloned and used to stimulate BMDCs. ► Mice receiving Com1 or Mip-pulsed DCs showed lower bacterial loads than control. ► Mice receiving Com1 or Mip-pulsed DCs exhibited greater IFN-γ-positive cells.