The relationship between the severity of radiation-induced oral mucositis and the myeloperoxidase levels in rats

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 120; no. 3; pp. 329 - 336
• Main Authors: Miyamoto, Hironori, DDS, Kanayama, Takeo, DDS, PhD, Horii, Koichiro, DDS, PhD, Kawai, Tatsuya, MD, PhD, Tsuchimochi, Tsukasa, DDS, PhD, Shigetomi, Toshio, DDS, PhD, Shibamoto, Yuta, MD, PhD, Shibuya, Yasuyuki, DDS, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2015
• Subjects: Animals; Dentistry; Dose-Response Relationship, Radiation; Female; Head and Neck Neoplasms - radiotherapy; Peroxidase - metabolism; Radiation Dosage; Radiation Injuries - etiology; Radiation Injuries - pathology; Rats; Rats, Inbred F344; Severity of Illness Index; Stomatitis - etiology; Stomatitis - pathology; Surgery; Tongue - radiation effects
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2015.04.012

Objective Oral mucositis is a common adverse reaction to radiotherapy for head and neck cancer, and there are concerns regarding a decreased quality of life in patients receiving radiotherapy. The purpose of this study was to investigate the relationship between the severity of radiation-induced oral mucositis and the myeloperoxidase (MPO) levels in irradiated tissues. Study Design Ninety-six F344 rats were divided into the following 4 groups: 10-Gy, 18-Gy, and 30-Gy irradiation groups, and a nonirradiation group. Oral mucositis was induced by the administration of single doses of radiation via exposure. After irradiation, the rats were evaluated on the basis of weight measurements, macroscopic findings according to a grading scale (Oral Mucositis Index [OMI]), and the results of tissue MPO assays. Results Weights decreased whereas the OMI scores and MPO levels increased, depending on the dose of exposure. The Spearman rank correlation test showed a significant correlation between the OMI scores and the MPO levels in the tissues with a correlation coefficient of 0.824 ( P  < .01). Conclusions In this study, the MPO levels in the irradiated tissue were increased in the cases involving severe radiation-induced oral mucositis evaluated in rats using a grading scale.