Chloroplast and nuclear microsatellite analysis of Aegilops cylindrica

• Published in: Theoretical and applied genetics Vol. 111; no. 3; pp. 561 - 572
• Main Authors: Gandhi, H.T, Vales, M.I, Watson, C.J.W, Mallory-Smith, C.A, Mori, N, Rehman, M, Zemetra, R.S, Riera-Lizarazu, O
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.08.2005; Berlin Springer Nature B.V
• Subjects: Aegilops cylindrica; Aegilops markgrafii; Aegilops tauschii; Alleles; Biological and medical sciences; Cell Nucleus - genetics; chloroplast DNA; Chloroplasts; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; DNA, Plant; Fundamental and applied biological sciences. Psychology; Genetic markers; Genetic Variation; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genome, Plant; heterozygosity; loci; Microsatellite Repeats; nuclear genome; Phylogeny; Poaceae - classification; Poaceae - genetics; Pteridophyta, spermatophyta; Triticum aestivum; Triticum turgidum subsp. durum; Vegetals; Monocotyledones; Aegilops; Gramineae; Analysis; Microsatellite DNA; Angiospermae; Genetics; Spermatophyta; Agriculture; Chloroplast
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-005-2047-z

Aegilops cylindrica Host (2n=4x=28, genome CCDD) is an allotetraploid formed by hybridization between the diploid species Ae. tauschii Coss. (2n=2x=14, genome DD) and Ae. markgrafii (Greuter) Hammer (2n=2x=14, genome CC). Previous research has shown that Ae. tauschii contributed its cytoplasm to Ae. cylindrica. However, our analysis with chloroplast microsatellite markers showed that 1 of the 36 Ae. cylindrica accessions studied, TK 116 (PI 486249), had a plastome derived from Ae. markgrafii rather than Ae. tauschii. Thus, Ae. markgrafii has also contributed its cytoplasm to Ae. cylindrica. Our analysis of chloroplast and nuclear microsatellite markers also suggests that D-type plastome and the D genome in Ae. cylindrica were closely related to, and were probably derived from, the tauschii gene pool of Ae. tauschii. A determination of the likely source of the C genome and the C-type plastome in Ae. cylindrica was not possible.