Chemokine requirements for B cell entry to lymph nodes and Peyer's patches

• Published in: The Journal of experimental medicine Vol. 196; no. 1; pp. 65 - 75
• Main Authors: Okada, Takaharu, Ngo, Vu N, Ekland, Eric H, Förster, Reinhold, Lipp, Martin, Littman, Dan R, Cyster, Jason G
• Format: Journal Article
• Language: English
• Published: United States The Rockefeller University Press 01.07.2002
• Subjects: Animals; B-Lymphocytes - cytology; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Cell Adhesion - immunology; Cell Movement - immunology; Chemokine CXCL12; Chemokine CXCL13; Chemokines - metabolism; Chemokines, CXC - genetics; Chemokines, CXC - metabolism; Endothelium, Vascular - cytology; Endothelium, Vascular - immunology; Endothelium, Vascular - metabolism; Lymph Nodes - cytology; Lymph Nodes - immunology; Mice; Mice, Inbred Strains; Mice, Mutant Strains; Peyer's Patches - cytology; Peyer's Patches - immunology; Receptors, CCR7; Receptors, Chemokine - antagonists & inhibitors; Receptors, Chemokine - metabolism; Receptors, CXCR4 - antagonists & inhibitors; Receptors, CXCR4 - metabolism; Receptors, CXCR5; Receptors, Cytokine - metabolism; Receptors, Lymphocyte Homing - metabolism; RNA, Messenger - metabolism; Signal Transduction - physiology; Venules
• ISSN: 0022-1007; 1540-9538
• DOI: 10.1084/jem.20020201

B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4-/- B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.