Polymorphic microsatellite loci isolated from the Squalidus argentatus using PCR-Based Isolation of Microsatellite Arrays (PIMA)

Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) is a small-sized freshwater fish which is distributed in Mainland China, Hainan Island and Taiwan. The populations of S. argentatus have dropped sharply probably due to overharvesting and water pollution recently. Eleven polymorphic microsat...

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Published inInternational journal of molecular sciences Vol. 12; no. 9; pp. 5666 - 5671
Main Authors Sun, Yang, Lin, Hung-Du, Tang, Wen-Qiao, Ju, Yu-Min, Liu, Zhi-Zhi, Liu, Dong, Yang, Jin-Quan
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.09.2011
Molecular Diversity Preservation International (MDPI)
Subjects
Alleles
Animals
China
Cloning
conservation
Cyprinidae - genetics
Gene Frequency
Genetic Loci - genetics
Genotype
Linkage Disequilibrium
microsatellite
Microsatellite Repeats - genetics
Oligonucleotide Array Sequence Analysis - methods
PIMA
Polymerase Chain Reaction - methods
Polymorphism
Polymorphism, Genetic
RAPD-PCR enrichment
Rivers
Squalidus argentatus
China
Taiwan
Hainan Island
PIMA
microsatellite
RAPD-PCR enrichment
conservation
Squalidus argentatus
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Summary:Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) is a small-sized freshwater fish which is distributed in Mainland China, Hainan Island and Taiwan. The populations of S. argentatus have dropped sharply probably due to overharvesting and water pollution recently. Eleven polymorphic microsatellite markers were developed for the cyprinid fish S. argentatus. These new markers were tested on 43 individuals collected from Yangtze River and Qiantang River. The number of alleles, observed and expected heterozygosity per locus, in two populations ranged from 3 to 14, from 0.333 to 0.954 and from 0.480 to 0.928, respectively. Only two loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency. No significant linkage disequilibrium was detected between the pairwise comparisons of these loci. These polymorphic microsatellite loci will enable us to study the genetic variation, population structure, and conservation genetics of this species in the future.
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These authors contributed equally to this work.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms12095666