Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

• Published in: Revista da Sociedade Brasileira de Medicina Tropical Vol. 46; no. 1; pp. 106 - 107
• Main Authors: Menezes, Everardo Albuquerque, Vasconcelos Júnior, Antônio Alexandre de, Ângelo, Maria Rozzelê Ferreira, Cunha, Maria da Conceição dos Santos Oliveira, Cunha, Francisco Afrânio
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Sociedade Brasileira de Medicina Tropical 01.01.2013; Sociedade Brasileira de Medicina Tropical - SBMT; Sociedade Brasileira de Medicina Tropical (SBMT)
• Subjects: Antifungal Agents - pharmacology; Antifungal susceptibility; Candida - classification; Candida - drug effects; Candida - isolation & purification; Disk diffusion; Epidemiology; Fluconazole; Fluconazole - pharmacology; Fungal infections; Humans; Microbial Sensitivity Tests - methods; TROPICAL MEDICINE; Fluconazole; Antifungal susceptibility; Disk diffusion
• ISSN: 0037-8682; 1678-9849; 1678-9849
• DOI: 10.1590/0037-868210502013

Antifungal susceptibility testing assists in finding the appropriate treatment for fungal infections, which are increasingly common. However, such testing is not very widespread. There are several existing methods, and the correlation between such methods was evaluated in this study. The susceptibility to fluconazole of 35 strains of Candida sp. isolated from blood cultures was evaluated by the following methods: microdilution, Etest, and disk diffusion. The correlation between the methods was around 90%. The disk diffusion test exhibited a good correlation and can be used in laboratory routines to detect strains of Candida sp. that are resistant to fluconazole.