Bacterial bioluminescence: Isolation and genetic analysis of functions from Vibrio fischeri
Recombinant E. coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri. All luminescent clones had a 16 kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expressio...
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Published in | Cell Vol. 32; no. 3; pp. 773 - 781 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.01.1983
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Subjects | |
Online Access | Get full text |
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Summary: | Recombinant E. coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium Vibrio fischeri. All luminescent clones had a 16 kb insert that encoded enzymatic activities for the light reaction as well as regulatory functions necessary for expression of the luminescence phenotype (Lux). Mutants generated by transposons Tn5 and mini-Mu were used to define Lux functions and to determine the genetic organization of the
lux region. Regulatory and enzymatic functions were assigned to regions of two
lux operons. With transcriptional fusions between the
lacZ gene on transposon mini-Mu and the target gene, expression of
lux operons could be measured in the absence of light production. The direction of transcription of
lux operons was deduced from the orientation of mini-Mu insertions in the fusion plasmids. Induction of transcription of one
lux operon required a function encoded by that operon (autoregulation). From these and other regulatory relationships, we propose a model for genetic control of light production. |
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Bibliography: | L L73 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/0092-8674(83)90063-6 |