Hedgehog signaling regulates the survival of gastric cancer cells by regulating the expression of Bcl-2

Gastric cancer is the second most common cause of cancer deaths worldwide. The underlying molecular mechanisms of its carcinogenesis are relatively poorly characterized. Hedgehog (Hh) signaling, which is critical for development of various organs including the gastrointestinal tract, has been associ...

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Published inInternational journal of molecular sciences Vol. 10; no. 7; pp. 3033 - 3043
Main Authors Han, Myoung-Eun, Lee, Young-Suk, Baek, Sun-Yong, Kim, Bong-Seon, Kim, Jae-Bong, Oh, Sae-Ock
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 06.07.2009
Molecular Diversity Preservation International (MDPI)
Subjects
Apoptosis
Apoptosis - drug effects
Bcl-2
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cytochrome
Flow cytometry
Gastric cancer
Gene Expression Regulation, Neoplastic
Hedgehog Proteins - genetics
Hedgehog Proteins - metabolism
hedgehog signaling
Humans
Medical prognosis
Prostate
Proteins
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
Signal Transduction - drug effects
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Veratrum Alkaloids - pharmacology
Bcl-2
apoptosis
gastric cancer
hedgehog signaling
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Summary:Gastric cancer is the second most common cause of cancer deaths worldwide. The underlying molecular mechanisms of its carcinogenesis are relatively poorly characterized. Hedgehog (Hh) signaling, which is critical for development of various organs including the gastrointestinal tract, has been associated with gastric cancer. The present study was undertaken to reveal the underlying mechanism by which Hh signaling controls gastric cancer cell proliferation. Treatment of gastric cancer cells with cyclopamine, a specific inhibitor of Hh signaling pathway, reduced proliferation and induced apoptosis of gastric cancer cells. Cyclopamine treatment induced cytochrome c release from mitochondria and cleavage of caspase 9. Moreover, Bcl-2 expression was significantly reduced by cyclopamine treatment. These results suggest that Hh signaling regulates the survival of gastric cancer cells by regulating the expression of Bcl-2.
Bibliography:These authors contributed equally to this work
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms10073033