Closing the Gaps in Pediatric Laboratory Reference Intervals: A CALIPER Database of 40 Biochemical Markers in a Healthy and Multiethnic Population of Children

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 58; no. 5; pp. 854 - 868
• Main Authors: Colantonio, David A, Kyriakopoulou, Lianna, Chan, Man Khun, Daly, Caitlin H, Brinc, Davor, Venner, Allison A, Pasic, Maria D, Armbruster, David, Adeli, Khosrow
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for Clinical Chemistry 01.05.2012; Oxford University Press
• Subjects: Adolescent; Age Factors; Analytical, structural and metabolic biochemistry; Architects; Asian Continental Ancestry Group; Biological and medical sciences; Biomarkers - blood; Canada; Child; Child, Preschool; Children & youth; Databases, Factual; Ethnicity; European Continental Ancestry Group; Female; Fundamental and applied biological sciences. Psychology; Gender differences; Hospitals; Humans; Hypothyroidism; Infant; Infant, Newborn; Investigative techniques, diagnostic techniques (general aspects); Male; Medical research; Medical sciences; Metabolic disorders; Molecular biophysics; Nutrition research; Pediatrics; Reference Values; Schools; Sex Factors; Canada; Human; Pediatrics; Reference value; Crosscultural study; Healthy subject; Biochemical marker; Biochemistry; Laboratory; Ethnic group; Clinical biology; Gap; Database; Molecular biology; Child
• ISSN: 0009-9147; 1530-8561; 1530-8561
• DOI: 10.1373/clinchem.2011.177741

Pediatric healthcare is critically dependent on the availability of accurate and precise laboratory biomarkers of pediatric disease, and on the availability of reference intervals to allow appropriate clinical interpretation. The development and growth of children profoundly influence normal circulating concentrations of biochemical markers and thus the respective reference intervals. There are currently substantial gaps in our knowledge of the influences of age, sex, and ethnicity on reference intervals. We report a comprehensive covariate-stratified reference interval database established from a healthy, nonhospitalized, and multiethnic pediatric population. Healthy children and adolescents (n = 2188, newborn to 18 years of age) were recruited from a multiethnic population with informed parental consent and were assessed from completed questionnaires and according to defined exclusion criteria. Whole-blood samples were collected for establishing age- and sex-stratified reference intervals for 40 serum biochemical markers (serum chemistry, enzymes, lipids, proteins) on the Abbott ARCHITECT c8000 analyzer. Reference intervals were generated according to CLSI C28-A3 statistical guidelines. Caucasians, East Asians, and South Asian participants were evaluated with respect to the influence of ethnicity, and statistically significant differences were observed for 7 specific biomarkers. The establishment of a new comprehensive database of pediatric reference intervals is part of the Canadian Laboratory Initiative in Pediatric Reference Intervals (CALIPER). It should assist laboratorians and pediatricians in interpreting test results more accurately and thereby lead to improved diagnosis of childhood diseases and reduced patient risk. The database will also be of global benefit once reference intervals are validated in transference studies with other analytical platforms and local populations, as recommended by the CLSI.