A tutorial on optical photothermal infrared (O-PTIR) microscopy

• Published in: APL photonics Vol. 9; no. 9; pp. 091101 - 091101-43
• Main Authors: Prater, Craig B., Kansiz, Mustafa, Cheng, Ji-Xin
• Format: Journal Article
• Language: English
• Published: United States AIP Publishing LLC 01.09.2024
• Subjects: Tutorials
• ISSN: 2378-0967; 2378-0967
• DOI: 10.1063/5.0219983

This tutorial reviews the rapidly growing field of optical photothermal infrared (O-PTIR) spectroscopy and chemical imaging. O-PTIR is an infrared super-resolution measurement technique where a shorter wavelength visible probe is used to measure and map infrared (IR) absorption with spatial resolution up to 30× better than conventional techniques such as Fourier transform infrared and direct IR laser imaging systems. This article reviews key limitations of conventional IR instruments, the O-PTIR technology breakthroughs, and their origins that have overcome the prior limitations. This article also discusses recent developments in expanding multi-modal O-PTIR approaches that enable complementary Raman spectroscopy and fluorescence microscopy imaging, including wide-field O-PTIR imaging with fluorescence-based detection of IR absorption. Various practical subjects are covered, including sample preparation techniques, optimal measurement configurations, use of IR tags/labels and techniques for data analysis, and visualization. Key O-PTIR applications are reviewed in many areas, including biological and biomedical sciences, environmental and microplastics research, (bio)pharmaceuticals, materials science, cultural heritage, forensics, photonics, and failure analysis.